激活沉默的阿尔戈英雄。
Activating silent argonautes.
出版信息
Nat Struct Mol Biol. 2013 Jul;20(7):769-71. doi: 10.1038/nsmb.2620.
PMID:23984440
Abstract
Multiple Argonaute proteins are implicated in gene silencing by RNA interference (RNAi), but only one is known to be an endonuclease that can cleave target mRNAs. Chimeric Argonaute proteins now reveal an unexpected mechanism by which mutations distal to the catalytic center can unmask intrinsic catalytic activity, results hinting at structurally mediated regulation.
摘要
多种 Argonaute 蛋白被牵涉到 RNA 干扰（RNAi）的基因沉默中，但只有一种已知是能够切割靶 mRNA 的内切核酸酶。嵌合 Argonaute 蛋白现在揭示了一种意想不到的机制，即远离催化中心的突变可以揭示内在的催化活性，这一结果暗示了结构介导的调控。
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