School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Proteomics. 2013 Jun;13(12-13):1871-84. doi: 10.1002/pmic.201200428.
Fruit tree crops are agricultural commodities of high economic importance, while fruits also represent one of the most vital components of the human diet. Therefore, a great effort has been made to understand the molecular mechanisms covering fundamental biological processes in fruit tree physiology and fruit biology. Thanks to the development of cutting-edge "omics" technologies such as proteomic analysis, scientists now have powerful tools to support traditional fruit tree research. Such proteomic analyses are establishing high-density 2DE reference maps and peptide mass fingerprint databases that can lead fruit science into a new postgenomic research era. Here, an overview of the application of proteomics in key aspects of fruit tree physiology as well as in fruit biology, including defense responses to abiotic and biotic stress factors, is presented. A panoramic view of ripening-related proteins is also discussed, as an example of proteomic application in fruit science.
果树作物是具有重要经济意义的农业商品，而水果也是人类饮食中最重要的组成部分之一。因此，人们付出了巨大的努力来了解覆盖果树生理学和果实生物学基本生物学过程的分子机制。得益于蛋白质组分析等前沿“组学”技术的发展，科学家们现在拥有了支持传统果树研究的强大工具。这些蛋白质组分析正在建立高密度 2DE 参考图谱和肽质量指纹数据库，这将使果实科学进入一个新的后基因组研究时代。本文综述了蛋白质组学在果树生理学和果实生物学关键方面的应用，包括对生物和非生物胁迫因素的防御反应。还讨论了与成熟相关的蛋白质的全景图，作为蛋白质组学在果实科学中应用的一个例子。
