Faculty of Science and Technology, University of Westminster, London, UK.
Aarhus University, Denmark.
Microb Biotechnol. 2017 Nov;10(6):1384-1399. doi: 10.1111/1751-7915.12782. Epub 2017 Sep 14.
This study demonstrated the utilization of unprocessed biodiesel waste as a carbon feedstock for Pseudomonas mendocinaCH50, for the production of PHAs. A PHA yield of 39.5% CDM was obtained using 5% (v/v) biodiesel waste substrate. Chemical analysis confirmed that the polymer produced was poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate) or P(3HHx-3HO-3HD-3HDD). P(3HHx-3HO-3HD-3HDD) was further characterized and evaluated for its use as a tissue engineering scaffold (TES). This study demonstrated that P(3HHx-3HO-3HD-3HDD) was biocompatible with the C2C12 (myoblast) cell line. In fact, the % cell proliferation of C2C12 on the P(3HHx-3HO-3HD-3HDD) scaffold was 72% higher than the standard tissue culture plastic confirming that this novel PHA was indeed a promising new material for soft tissue engineering.
本研究展示了将未经处理的生物柴油废料作为碳源用于假单胞菌 CH50 生产 PHAs 的利用。使用 5%（v/v）生物柴油废料基质可获得 39.5%CDM 的 PHAs 产率。化学分析证实所生产的聚合物是聚（3-羟基己酸-co-3-羟基辛酸-co-3-羟基癸酸-co-3-羟基十二酸）或 P（3HHx-3HO-3HD-3HDD）。进一步对 P（3HHx-3HO-3HD-3HDD）进行了表征和评估，以将其用作组织工程支架（TES）。本研究表明，P（3HHx-3HO-3HD-3HDD）与 C2C12（成肌细胞）细胞系具有生物相容性。事实上，C2C12 在 P（3HHx-3HO-3HD-3HDD）支架上的细胞增殖率比标准组织培养塑料高 72%，这证实了这种新型 PHAs 确实是软组织工程的一种有前途的新材料。
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