Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Carbohydr Polym. 2013 Oct 15;98(1):428-37. doi: 10.1016/j.carbpol.2013.06.011. Epub 2013 Jun 19.
Water-soluble corn silk polysaccharides (CSPS) were chemically modified to obtain their sulfated, acetylated and carboxymethylated derivatives. Chemical characterization and bioactivities of CSPS and its derivatives were comparatively investigated by chemical methods, gas chromatography, gel filtration chromatography, scanning electron microscope, infrared spectroscopy and circular dichroism spectroscopy, scavenging DPPH free radical assay, scavenging hydroxyl radical assay, ferric reducing power assay, lipid peroxidation inhibition assay and α-amylase activity inhibitory assay, respectively. Among the three derivatives, carboxylmethylated polysaccharide (C-CSPS) demonstrated higher solubility, narrower molecular weight distribution, lower intrinsic viscosity, a hyperbranched conformation, significantly higher antioxidant and α-amylase inhibitory abilities compared with the native polysaccharide and other derivatives. C-CSPS might be used as a novel nutraceutical agent for human consumption.
水溶性玉米须多糖（CSPS）经化学修饰得到其硫酸酯、乙酰化和羧甲基化衍生物。通过化学方法、气相色谱、凝胶过滤色谱、扫描电子显微镜、红外光谱和圆二色谱对 CSPS 及其衍生物的化学性质和生物活性进行了比较研究，分别采用 DPPH 自由基清除测定法、羟基自由基清除测定法、铁还原能力测定法、脂质过氧化抑制测定法和α-淀粉酶活性抑制测定法。在这三种衍生物中，与天然多糖和其他衍生物相比，羧甲基化多糖（C-CSPS）具有更高的溶解度、更窄的分子量分布、更低的特性粘度、超支化构象，具有更高的抗氧化和α-淀粉酶抑制能力。C-CSPS 可能被用作人类消费的新型营养药物。
