Diers Anne R, Keszler Agnes, Hogg Neil
Department of Biophysics and Redox Biology Program, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Biochim Biophys Acta. 2014 Feb;1840(2):892-900. doi: 10.1016/j.bbagen.2013.07.026. Epub 2013 Aug 27.
S-nitrosothiols have been recognized as biologically-relevant products of nitric oxide that are involved in many of the diverse activities of this free radical.
This review serves to discuss current methods for the detection and analysis of protein S-nitrosothiols. The major methods of S-nitrosothiol detection include chemiluminescence-based methods and switch-based methods, each of which comes in various flavors with advantages and caveats.
The detection of S-nitrosothiols is challenging and prone to many artifacts. Accurate measurements require an understanding of the underlying chemistry of the methods involved and the use of appropriate controls.
Nothing is more important to a field of research than robust methodology that is generally trusted. The field of S-nitrosation has developed such methods but, as S-nitrosothiols are easy to introduce as artifacts, it is vital that current users learn from the lessons of the past. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.
S-亚硝基硫醇被认为是一氧化氮的生物相关产物，参与了这种自由基的许多不同活动。
本综述旨在讨论蛋白质S-亚硝基硫醇检测和分析的当前方法。S-亚硝基硫醇检测的主要方法包括基于化学发光的方法和基于开关的方法，每种方法都有多种形式，各有优缺点。
S-亚硝基硫醇的检测具有挑战性，容易产生许多假象。准确的测量需要了解所涉及方法的基础化学知识并使用适当的对照。
对于一个研究领域来说，没有什么比普遍值得信赖的可靠方法更重要了。亚硝基化领域已经开发出了这样的方法，但是，由于S-亚硝基硫醇很容易作为假象引入，当前的使用者从过去的经验中吸取教训至关重要。本文是名为“研究活性氧物种的当前方法——优缺点及膜蛋白生物物理学”的特刊的一部分。客座编辑：克里斯汀·温特伯恩。
