Seckler James M, Meyer Nikki M, Burton Spencer T, Bates James N, Gaston Benjamin, Lewis Stephen J
Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, United States of America.
Department of Anesthesia, University of Iowa, Iowa City, Iowa, United States of America.
PLoS One. 2017 Oct 26;12(10):e0187149. doi: 10.1371/journal.pone.0187149. eCollection 2017.
Small molecule S-nitrosothiols are a class of endogenous chemicals in the body, which have been implicated in a variety of biological functions. However, the labile nature of NO and the limits of current detection assays have made studying these molecules difficult. Here we present a method for detecting trace concentrations of S-nitrosothiols in biological fluids. Capacitive sensors when coupled to a semiconducting material represent a method for detecting trace quantities of a chemical in complex solutions. We have taken advantage of the semiconducting and chemical properties of polydopamine to construct a capacitive sensor and associated method of use, which specifically senses S-nitrosothiols in complex biological solutions.
小分子硫代亚硝基化合物是体内一类内源性化学物质，它们与多种生物学功能有关。然而，一氧化氮（NO）的不稳定性质以及当前检测方法的局限性使得对这些分子的研究变得困难。在此，我们提出一种检测生物体液中痕量硫代亚硝基化合物的方法。电容式传感器与半导体材料结合时，是一种检测复杂溶液中痕量化学物质的方法。我们利用聚多巴胺的半导体和化学性质构建了一种电容式传感器及其相关使用方法，该方法能够特异性地检测复杂生物溶液中的硫代亚硝基化合物。
