分离并优化一种新型破囊壶菌菌株，用于生产富含二十二碳六烯酸（DHA）和虾青素的生物质，作为水产饲料添加剂。
Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement.
作者信息
Pawar Pratik R, Velani Sneha, Kumari Sujata, Lali Arvind M, Prakash Gunjan
机构信息
DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.
Department of Chemical Engineering, Institute of Chemical Technology, Mumbai, India.
出版信息
3 Biotech. 2021 Feb;11(2):71. doi: 10.1007/s13205-020-02616-4. Epub 2021 Jan 13.
Abstract
A marine organism, belonging to the Thraustochytrids family was isolated from mangroves of Mumbai, India. The isolated strain was identified as by internal transcribed spacer sequence analysis Optimization of process parameters yielded 14.47 g/L dry cell weight containing 55-58% oil in 3.5 days' cultivation on glucose, yeast extract, and peptone in the bioreactor. Docosahexaenoic acid (DHA) was found to be the dominant long-chain polyunsaturated fatty acid, accounting for 32-35% of total fatty acid content. The process parameter was tweaked to simultaneously synthesize astaxanthin along with DHA. The concurrent synthesis of DHA and astaxanthin-containing biomass establishes the isolated strain as a perfect choice for aquafeed. : NCBI accession number MN046792.
摘要
一种属于破囊壶菌科的海洋生物从印度孟买的红树林中分离出来。通过内部转录间隔区序列分析鉴定出分离菌株。在生物反应器中，以葡萄糖、酵母提取物和蛋白胨为培养基，经过3.5天的培养，优化工艺参数后得到了干细胞重量为14.47 g/L且含油55 - 58%的产物。发现二十二碳六烯酸（DHA）是主要的长链多不饱和脂肪酸，占总脂肪酸含量的32 - 35%。对工艺参数进行调整以同时合成虾青素和DHA。DHA与含虾青素生物量的同时合成表明该分离菌株是水产饲料的理想选择。：NCBI登录号MN046792 。
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