Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-Dong, Yuseong, Daejeon 305-764, Republic of Korea.
Korea Research Institute of Bioscience & Biotechnology, 52 Oun-Dong, Yuseong, Daejeon 305-333, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4730-4734. doi: 10.1099/ijs.0.054619-0. Epub 2013 Aug 29.
Two Gram-stain-positive, non-motile, non-spore-forming, rod-shaped actinobacterial strains were isolated from the surface-sterilized roots of mugwort (Artemisia princeps) and horse-weed (Conyza canadensis), and subjected to taxonomic characterization. 16S rRNA gene sequence analysis indicated that the isolates, designated MWE 3-5(T) and HWE 2-02(T), should be placed in the genus Nocardioides of the family Nocardioidaceae. The strains were closely related to Nocardioides hankookensis DS-30(T), which exhibited 16S rRNA gene sequence similarity values of 97.99 and 99.09 % with strains MWE 3-5(T) and HWE 2-02(T), respectively. The genome relatedness of N. hankookensis DS-30(T) with strain MWE 3-5(T) was 35.8 %, and that with strain HWE 2-02(T) was 36.4 %, whereas that between the two isolates was 43.2 %. Strains MWE 3-5(T) and HWE 2-02(T) possessed MK-8(H4) as the major isoprenoid quinone, and ll-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids were iso-C16 : 0, iso-C17 : 0 and C18 : 1ω9c for strain MWE 3-5(T) and iso-C16 : 0, 10-methyl C18 : 0 and C18 : 1ω9c for strain HWE 2-02(T). Based on phenotypic, genotypic and phylogenetic studies, the following two novel species are proposed: Nocardioides endophyticus sp. nov. (type strain, MWE 3-5(T) = KCTC 29122(T) = JCM 18532(T)) and Nocardioides conyzicola sp. nov. (type strain, HWE 2-02(T) = KCTC 29121(T) = JCM 18531(T)).
两株革兰氏阳性、非运动、非孢子形成、杆状放线菌菌株分别从艾蒿（Artemisia princeps）和鬼针草（Conyza canadensis）的表面消毒根部分离得到，并进行了分类学描述。16S rRNA 基因序列分析表明，这些分离株被命名为 MWE 3-5(T)和 HWE 2-02(T)，应属于诺卡氏菌科的诺卡氏菌属。这些菌株与 Nocardioides hankookensis DS-30(T)密切相关，其 16S rRNA 基因序列相似度分别为 97.99%和 99.09%。N. hankookensis DS-30(T)与 MWE 3-5(T)的基因组相关性为 35.8%，与 HWE 2-02(T)的基因组相关性为 36.4%，而两株菌株之间的相关性为 43.2%。MWE 3-5(T)和 HWE 2-02(T)菌株的主要异戊烯醌为 MK-8(H4)，细胞壁肽聚糖中含有 ll-二氨基庚二酸。主要脂肪酸为 MWE 3-5(T)菌株的 iso-C16:0、iso-C17:0 和 C18:1ω9c，HWE 2-02(T)菌株的 iso-C16:0、10-甲基 C18:0 和 C18:1ω9c。基于表型、基因型和系统发育研究，提出了以下两个新种：内生诺卡氏菌（模式株，MWE 3-5(T)=KCTC 29122(T)=JCM 18532(T)）和鬼针草诺卡氏菌（模式株，HWE 2-02(T)=KCTC 29121(T)=JCM 18531(T)）。
