Nocardioides daecheongensis sp. nov., isolated from soil.

Strain KIS2-16(T) was isolated from a soil sample collected from Daecheong Island of Incheon region, South Korea. KIS2-16(T) was Gram-staining-positive, aerobic, non-spore-forming, non-motile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16(T) represented a member of the genus Nocardioides, being most closely related to the type strains of species of the genus Nocardioides, Nocardioides maradonensis RP-B30(T) (97.8 % sequence similarity) and Nocardioides ultimimeridianus RP-B26(T) (97.0 %). The fatty acid profile of KIS2-16(T) was dominated by C18 : 1ω9c, C17 : 1ω8c, C16 : 0, C18 : 0 10-methyl (TBSA), C16 : 0 2-OH and C17 : 0 2-OH. The major isoprenoid quinone was MK-8(H4), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3γ-type with ll-diaminopimelic acid. The genomic DNA G+C content of KIS2-16(T) was 64.9 mol%. Strain KIS2-16(T) showed DNA-DNA hybridization values of less than 70 % with the closely related species of the genus Nocardioides. Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus Nocardioides, for which the name Nocardioides daecheongensis sp. nov. (type strain KIS2-16(T) = DSM 27136(T) = KACC 17297(T) = NBRC 109597(T)) is proposed.