Hur Hyun
Department of Biology, Dongguk University, Seoul 100-715, Korea.
Mycobiology. 2008 Jun;36(2):81-7. doi: 10.4489/MYCO.2008.36.2.081. Epub 2008 Jun 30.
The optimal conditions for mycelial growth of Phellinus linteus ATCC 26710 were determined to be a log length of 20 cm, temperature of 30℃ and pH of 6.0. Mycelial growth was excellent on the mushroom complete medium, and was optimal when sucrose, mannose and glucose were supplied as carbon sources. Potassium nitrate and sodium nitrate as nitrogen sources supported good mycelial growth. To evaluate P. linteus mycelial colonization on logs, sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation techniques were used. Only sterilized short log inoculation produced good mycelial colonization. Initial mycelial growth and full mycelial colonization were best on 20 cm logs having 42% moisture content. The initial mycelial growth of P. linteus was accelerated over 12 hr of sterilization. Basidiocarp formation was optimal using a burying method of logs after 56 months, and fruiting body formation was superior in cultivation house conditions of 3135℃ and in excess of 96% relative humidity.
桑黄ATCC 26710菌丝体生长的最佳条件为：段木长度20厘米、温度30℃、pH值6.0。在蘑菇全营养培养基上菌丝体生长良好，以蔗糖、甘露糖和葡萄糖作为碳源时生长最佳。以硝酸钾和硝酸钠作为氮源时，菌丝体生长良好。为评估桑黄在段木上的菌丝定殖情况，采用了灭菌短段木接种、打孔接种和段木末端夹心接种技术。只有灭菌短段木接种产生了良好的菌丝定殖。初始菌丝体生长和完全菌丝定殖在含水量42%的20厘米段木上最佳。桑黄的初始菌丝体生长在灭菌12小时后加速。采用段木掩埋法，5至6个月后子实体形成最佳，在31至35℃且相对湿度超过96%的栽培室条件下子实体形成更好。
