Alam Nuhu, Shim Mi Ja, Lee Min Woong, Shin Pyung Gyun, Yoo Young Bok, Lee Tae Soo
Department of Biology, University of Incheon, Incheon 406-840, Korea.
Mycobiology. 2009 Dec;37(4):258-66. doi: 10.4489/MYCO.2009.37.4.258. Epub 2009 Dec 31.
Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at 30℃ and minimum mycelial growth observed at 10℃. This mushroom tolerates a broad pH range for mycelial growth, with most favorable growth observed at pH 6. Results also indicated that glucose peptone, yeast malt extract and mushroom complete media were favorable growth media, while Hennerberg and Hoppkins media were unfavorable. Dextrin was the best and xylose the least effective carbon sources. Results revealed that inorganic nitrogen sources were less effective than organic sources for the mycelial growth of P. eryngii. Investigation of genetic diversity is necessary to identify the strains. The ITS region of rDNA were amplified using PCR. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 214 to 222 bp and 145 to 236 bp, respectively. The sequence of ITS2 was more variable than that of ITS1, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into six clusters. Fourteen IUM and ATCC-90212 strains were also analyzed by RAPD with 20 arbitrary primers. Fourteen of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.2 to 2.3 kb.
杏鲍菇，又称平菇王，已被广泛用于营养和药用目的。本研究旨在筛选适合菌丝体生长的条件，并确定所选菌株的系统发育关系。在30℃时观察到菌丝体生长最佳，在10℃时观察到菌丝体生长最少。这种蘑菇在较宽的pH范围内都能耐受菌丝体生长，在pH 6时观察到最有利的生长。结果还表明，葡萄糖蛋白胨、酵母麦芽提取物和蘑菇完全培养基是有利的生长培养基，而亨纳伯格和霍普金斯培养基则不利。糊精是最好的碳源，木糖是最无效的碳源。结果表明，无机氮源对杏鲍菇菌丝体生长的效果不如有机氮源。有必要对菌株进行遗传多样性研究。使用PCR扩增rDNA的ITS区域。不同菌株rDNA的ITS1和ITS2区域大小分别在214至222 bp和145至236 bp之间变化。ITS2的序列比ITS1的序列更具变异性，且5.8S序列相同。基于ITS区域序列的系统发育树表明，所选菌株可分为六个簇。还使用20个任意引物通过RAPD对14个IUM和ATCC - 90212菌株进行了分析。其中14个引物有效地扩增了基因组DNA。扩增条带的数量因引物和菌株而异，多态性片段范围在0.2至2.3 kb之间。
