Forim Moacir Rossi, Costa Eveline Soares, da Silva Maria Fátima das Graças Fernandes, Fernandes João Batista, Mondego Janaina Marques, Boiça Junior Arlindo Leal
Department of Chemistry, Federal University of São Carlos , Rod. Washington Luiz, Km 235, PB 676, São Carlos, SP 13.565-905, Brazil.
J Agric Food Chem. 2013 Sep 25;61(38):9131-9. doi: 10.1021/jf403187y. Epub 2013 Sep 12.
Biodegradable nanoparticles have been widely explored as carriers for controlled delivery of therapeutic molecules; however, studies describing the development of nanoparticles as carriers for biopesticide products are few. In this work, a new method to prepare nanoparticles loaded with neem (Azadirachta indica) extracts is presented. In this study, nanoparticles were formulated as colloidal suspension and (spray-dried) powder and characterized by evaluating pH, particle size, zeta potential, morphology, absolute recovery, and entrapment efficiency. A high-performance liquid chromatography method was used for nanoparticle characterization. The best formulations presented absolute recovery and entrapment efficiencies of approximately 100% and a release profile based on swelling and relaxation of the polymer or polymer erosion. The biological data of the formulated products against Plutella xylostella showed 100% larval mortality. The nanoparticle information improved the stability of neem products against ultraviolet radiation and increased their dispersion in the aqueous phase.
可生物降解的纳米颗粒已被广泛研究作为治疗分子控释的载体；然而，将纳米颗粒开发为生物农药产品载体的研究却很少。在这项工作中，提出了一种制备负载印楝（Azadirachta indica）提取物的纳米颗粒的新方法。在本研究中，纳米颗粒被制成胶体悬浮液和（喷雾干燥）粉末，并通过评估pH值、粒径、zeta电位、形态、绝对回收率和包封率进行表征。采用高效液相色谱法对纳米颗粒进行表征。最佳配方的绝对回收率和包封率约为100%，其释放曲线基于聚合物的溶胀和松弛或聚合物侵蚀。所制备产品对小菜蛾的生物学数据显示幼虫死亡率为100%。纳米颗粒信息提高了印楝产品对紫外线辐射的稳定性，并增加了它们在水相中的分散性。
