克什米尔地区白细胞介素8基因3'-非翻译区常见多态性与非小细胞肺癌的关联性缺失
Lack of association of a common polymorphism in the 3' -UTR of interleukin 8 with non small cell lung cancer in Kashmir.
作者信息
Bhat Imtiyaz Ahmad, Pandith Arshid A, Bhat Bashir A, Naykoo Niyaz A, Qasim Iqbal, Rasool Roohi, Aziz Sheik Aejaz, Shah Zafar Amin
机构信息
Department of Immunology and Molecular Medicine, Sher i Kashmir institute of Medical Sciences Srinagar, India.
出版信息
Asian Pac J Cancer Prev. 2013;14(7):4403-8. doi: 10.7314/apjcp.2013.14.7.4403.
BACKGROUND
Chronic inflammation is considered as an important factor in the pathogenesis of lung cancer. The presence of inflammatory cells and higher levels of pro-inflammatory cytokines in the tumor microenvironment and their surrounding tissues is gaining much importance in research.
MATERIALS AND METHODS
One hundred ninety NSCLC cases and 200 age, smoking and sex matched controls were evaluated for association of IL-8 -251 (rs4073) and IL-8 -845 (rs2227532) in our population. Restriction fragment length polymorphism (RFLP) was used followed by direct sequencing for the detection of SNPs.
RESULTS
The IL-8 -845 polymorphism was not found in our population. No significant association was observed between the IL-8 -251 AT genotypes and IL-8 -25 AA genotypes and NSCLC (p=0.05) in our population. The IL-8 -251 A allele was also non-significant (p=0.05) in NSCLC patients.
CONCLUSIONS
In conclusion, this report reveals lack of association between IL-8 - 251 A/T polymorphism and NSCLC in our Kashmir Valley population.
背景
慢性炎症被认为是肺癌发病机制中的一个重要因素。肿瘤微环境及其周围组织中炎症细胞的存在和促炎细胞因子水平的升高在研究中越来越受到重视。
材料与方法
对190例非小细胞肺癌（NSCLC）病例和200例年龄、吸烟情况及性别相匹配的对照者进行评估，以研究白细胞介素-8（IL-8）-251（rs4073）和IL-8 -845（rs2227532）在我们人群中的关联性。采用限制性片段长度多态性（RFLP）方法，随后进行直接测序以检测单核苷酸多态性（SNP）。
结果
在我们的人群中未发现IL-8 -845多态性。在我们的人群中，未观察到IL-8 -251 AT基因型和IL-8 -25 AA基因型与非小细胞肺癌之间存在显著关联（p = 0.05）。IL-8 -251 A等位基因在非小细胞肺癌患者中也无显著意义（p = 0.05）。
结论
总之，本报告揭示了在我们克什米尔山谷人群中，IL-8 - 251 A/T多态性与非小细胞肺癌之间缺乏关联性。
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