Bhat Imtiyaz Ahmad, Pandith Arshid A, Bhat Bashir A, Naykoo Niyaz A, Qasim Iqbal, Rasool Roohi, Aziz Sheik Aejaz, Shah Zafar Amin
Department of Immunology and Molecular Medicine, Sher i Kashmir institute of Medical Sciences Srinagar, India.
Asian Pac J Cancer Prev. 2013;14(7):4403-8. doi: 10.7314/apjcp.2013.14.7.4403.
Chronic inflammation is considered as an important factor in the pathogenesis of lung cancer. The presence of inflammatory cells and higher levels of pro-inflammatory cytokines in the tumor microenvironment and their surrounding tissues is gaining much importance in research.
One hundred ninety NSCLC cases and 200 age, smoking and sex matched controls were evaluated for association of IL-8 -251 (rs4073) and IL-8 -845 (rs2227532) in our population. Restriction fragment length polymorphism (RFLP) was used followed by direct sequencing for the detection of SNPs.
The IL-8 -845 polymorphism was not found in our population. No significant association was observed between the IL-8 -251 AT genotypes and IL-8 -25 AA genotypes and NSCLC (p=0.05) in our population. The IL-8 -251 A allele was also non-significant (p=0.05) in NSCLC patients.
In conclusion, this report reveals lack of association between IL-8 - 251 A/T polymorphism and NSCLC in our Kashmir Valley population.
慢性炎症被认为是肺癌发病机制中的一个重要因素。肿瘤微环境及其周围组织中炎症细胞的存在和促炎细胞因子水平的升高在研究中越来越受到重视。
对190例非小细胞肺癌（NSCLC）病例和200例年龄、吸烟情况及性别相匹配的对照者进行评估，以研究白细胞介素-8（IL-8）-251（rs4073）和IL-8 -845（rs2227532）在我们人群中的关联性。采用限制性片段长度多态性（RFLP）方法，随后进行直接测序以检测单核苷酸多态性（SNP）。
在我们的人群中未发现IL-8 -845多态性。在我们的人群中，未观察到IL-8 -251 AT基因型和IL-8 -25 AA基因型与非小细胞肺癌之间存在显著关联（p = 0.05）。IL-8 -251 A等位基因在非小细胞肺癌患者中也无显著意义（p = 0.05）。
总之，本报告揭示了在我们克什米尔山谷人群中，IL-8 - 251 A/T多态性与非小细胞肺癌之间缺乏关联性。
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