印度南部特伦甘纳邦人群中肿瘤坏死因子α、干扰素γ和白细胞介素10基因多态性与非小细胞肺癌风险的关联
Association of TNFα, IFNγ, and IL10 gene polymorphisms and the risk of non-small cell lung cancer in the population of the South Indian state of Telangana.
作者信息
Peddireddy Vidyullatha, Badabagni Siva Prasad, Sulthana Shehnaz, Kolla Venkata Karunakar, Gundimeda Sandhya Devi, Mundluru Hemaprasad
机构信息
Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India.
Department of Biotechnology and Bioinformatics, University of Hyderabad, Gachibowli, Hyderabad, 500046, Telangana, India.
出版信息
Int J Clin Oncol. 2016 Oct;21(5):843-852. doi: 10.1007/s10147-016-0972-2. Epub 2016 Apr 16.
BACKGROUND
Cytokine-mediated inflammation is important in the pathogenesis of non-small cell lung cancer (NSCLC). Genetic polymorphisms in cytokine genes and their association with lung cancer in the Indian population have not been reported.
METHODS
For the first time, we analyzed genetic polymorphisms of TNFα , IFNγ , and IL10 genes in 246 NSCLC patients and 250 healthy controls in the South Indian population from Telangana using ARMS PCR.
RESULTS
IFNγ A/T and IL10 G/G gene polymorphisms were found to be significantly associated with NSCLC with 1.56- and 1.68-fold disease risk, respectively. There was no association between the risk of NSCLC and TNFα polymorphism. Gene polymorphisms stratified according to smoking revealed that IFNγ A/T polymorphisms in smokers increased the disease risk by 2.91 fold. IL10 G/G polymorphisms showed 2-fold increased risk among patients who were smokers when compared to the controls. However, there was no association between TNFα, IFNγ, and IL10 gene polymorphism and the stage of the NSCLC patients. The overall risk associated with the combination of these polymorphisms indicated that the TNFα G/A + IFNγ A/T + IL10 G/G genotype increased the risk by 1.5 fold.
CONCLUSIONS
The results of our study indicate an association between cytokine gene polymorphisms and the risk of NSCLC in an Indian population.
背景
细胞因子介导的炎症在非小细胞肺癌（NSCLC）的发病机制中起重要作用。细胞因子基因的遗传多态性及其与印度人群肺癌的关联尚未见报道。
方法
我们首次使用扩增阻滞突变系统聚合酶链反应（ARMS PCR）分析了来自印度特伦甘纳邦的246例NSCLC患者和250例健康对照者中肿瘤坏死因子α（TNFα）、干扰素γ（IFNγ）和白细胞介素10（IL10）基因的遗传多态性。
结果
发现IFNγ A/T和IL10 G/G基因多态性与NSCLC显著相关，疾病风险分别增加1.56倍和1.68倍。NSCLC风险与TNFα多态性之间无关联。根据吸烟情况分层的基因多态性显示，吸烟者中IFNγ A/T多态性使疾病风险增加2.91倍。与对照组相比，IL10 G/G多态性在吸烟患者中的风险增加2倍。然而，TNFα、IFNγ和IL10基因多态性与NSCLC患者的分期之间无关联。这些多态性组合的总体风险表明，TNFα G/A + IFNγ A/T + IL10 G/G基因型使风险增加1.5倍。
结论
我们的研究结果表明细胞因子基因多态性与印度人群NSCLC风险之间存在关联。
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