Dipartimento di Farmacia, Università di Napoli Federico II, Via D. Montesano 49, I-80131 Naples, Italy.
J Food Prot. 2013 Sep;76(9):1590-6. doi: 10.4315/0362-028X.JFP-13-054.
The presence of five bisphenols, i.e., bisphenol F, bisphenol A, bisphenol B, bisphenol F diglycidyl ether, and bisphenol A diglycidyl ether, was monitored in commercial milk packed in plastic bottles marketed in Italy. The new validated method includes a solid-phase extraction procedure followed by liquid chromatography with fluorescence detection. All positive results were confirmed by liquid chromatography-tandem mass spectrometry analysis. The limits of detection and quantification and the recovery percentages indicated that the method is suitable for detecting bisphenols in milk at concentrations far below the legal limits. Of 68 commercial milk samples analyzed, no bisphenol was found in 27 samples (39.7%), and 41 samples (60.3%) contained one or more bisphenols. The bisphenol most frequently found was bisphenol F (36 samples, 52.9%) followed by bisphenol A (20 samples, 29.4%) and bisphenol B (6 samples, 8.8%). Taking into consideration the limits of detection, no sample contained either bisphenol F diglycidyl ether or bisphenol A diglycidyl ether.
五种双酚，即双酚 F、双酚 A、双酚 B、双酚 F 二缩水甘油醚和双酚 A 二缩水甘油醚，在意大利市场上销售的塑料瓶装商业牛奶中被监测到。新验证的方法包括固相萃取程序，随后是带有荧光检测的液相色谱。所有阳性结果均通过液相色谱-串联质谱分析得到确认。检测限、定量限和回收率百分比表明，该方法适用于检测牛奶中浓度远低于法定限量的双酚。在分析的 68 个商业牛奶样本中，27 个样本（39.7%）未检出双酚，41 个样本（60.3%）含有一种或多种双酚。最常发现的双酚是双酚 F（36 个样本，52.9%），其次是双酚 A（20 个样本，29.4%）和双酚 B（6 个样本，8.8%）。考虑到检测限，没有样本含有双酚 F 二缩水甘油醚或双酚 A 二缩水甘油醚。
