一种快速、简单的 HPLC-FLD 筛选方法，采用 QuEChERS 作为样品前处理，可同时监测牛奶中的 9 种双酚。

A rapid and simple HPLC-FLD screening method with QuEChERS as the sample treatment for the simultaneous monitoring of nine bisphenols in milk.

机构信息

Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050 Gansu, China; Laboratory of Quality & Safety Risk Assessment for Livestock Product (Lanzhou), Ministry of Agriculture, Lanzhou 730050 Gansu, China; Key Laboratory for Yak Genetics, Breeding & Reproduction Engineering of Gansu Province, Lanzhou 730050 Gansu, China.

出版信息

Food Chem. 2018 Apr 1;244:371-377. doi: 10.1016/j.foodchem.2017.10.030. Epub 2017 Oct 10.

DOI:10.1016/j.foodchem.2017.10.030

PMID:29120796

Abstract

A specific, precise and accurate high performance liquid chromatographic (HPLC) analytical method with a fluorescence detector (FLD) was established for the simultaneous determination of nine bisphenols (BPs) in milk samples. Samples were extracted ultrasonically with acetonitrile and cleaned using the QuEChERS technique. Under the optimized conditions, good linearity was obtained for the nine BPs and the correlation coefficients (R) ranged from 0.9942 to 0.9997. Recovery values for the nine bisphenols in spiked samples were 75.82-93.86% with intra-day and inter-day relative standard deviations (RSDs) from 2.6 to 11.1%. The limits of detection (LODs) and quantification (LOQs) were 1.0-3.1 μg/kg and 3.5-9.8 μg/kg, respectively. The results demonstrated clearly that the approach developed provides reliable, simple, rapid and environmentally-friendly quantification and identification of nine bisphenols in a fatty matrix and could be used for monitoring bisphenols in milk.

摘要

建立了一种同时测定牛奶样品中九种双酚（BP）的特定、精确和准确的高效液相色谱（HPLC）荧光检测（FLD）分析方法。样品用乙腈超声提取，并用 QuEChERS 技术净化。在优化条件下，九种 BP 均获得良好的线性关系，相关系数（R）范围为 0.9942 至 0.9997。九种双酚在加标样品中的回收率为 75.82-93.86%，日内和日间相对标准偏差（RSD）为 2.6-11.1%。检测限（LOD）和定量限（LOQ）分别为 1.0-3.1μg/kg 和 3.5-9.8μg/kg。结果表明，该方法提供了一种可靠、简单、快速和环保的方法，可用于测定脂肪基质中九种双酚的含量，并可用于监测牛奶中的双酚。