鉴定参与海鞘素生物合成的一个甲基转移酶。
Characterization of a methyltransferase involved in herboxidiene biosynthesis.
机构信息
Department of Applied Chemistry and Biological Engineering, College of Chemical Engineering, Northeast Dianli University, Jilin, Jilin 132012, China; Department of Biological Engineering, Utah State University, 4105 Old Main Hill, Logan, UT 84322, United States.
出版信息
Bioorg Med Chem Lett. 2013 Oct 15;23(20):5667-70. doi: 10.1016/j.bmcl.2013.08.023. Epub 2013 Aug 13.
The herboxidiene biosynthetic gene cluster contains a regulatory gene and six biosynthetic genes that encode three polyketide synthases (HerB, HerC and HerD) and three tailoring enzymes (HerE, HerF and HerG). Through single crossover recombination, an integrative plasmid was inserted into the genome of Streptomyces chromofuscus ATCC 49982 between herE and herF, resulting in low-level expression of herF and the downstream herG. The mutant strain produced two new compounds, 18-deoxy-25-demethyl-herboxidiene and 25-demethyl-herboxidiene. HerF was expressed in Escherichia coli and biochemically characterized as the dedicated methyltransferase in herboxidiene biosynthesis. It prefers 25-demethyl-herboxidiene to 18-deoxy-25-demethyl-herboxidiene, suggesting that C-25 methylation is the last tailoring step.
赫罗巴烯生物合成基因簇包含一个调控基因和六个生物合成基因，编码三个聚酮合酶（HerB、HerC 和 HerD）和三个修饰酶（HerE、HerF 和 HerG）。通过单交换重组，整合质粒被插入到链霉菌属变色亚种 ATCC 49982 的基因组中，位于 herE 和 herF 之间，导致 herF 和下游 herG 的低水平表达。突变株产生了两种新化合物，18-脱氧-25-去甲基-赫罗巴烯和 25-去甲基-赫罗巴烯。HerF 在大肠杆菌中表达，并被生物化学表征为赫罗巴烯生物合成中的专用甲基转移酶。它更喜欢 25-去甲基-赫罗巴烯而不是 18-脱氧-25-去甲基-赫罗巴烯，表明 C-25 甲基化是最后一步修饰。
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