Jin Ming Ji, Kim In Sook, Rehman Shaheed Ur, Dong Mi-Sook, Na Chun-Soo, Yoo Hye Hyun
Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
J Chromatogr Sci. 2016 Mar;54(3):390-6. doi: 10.1093/chromsci/bmv152. Epub 2015 Oct 24.
The purpose of this study was to develop a simultaneous method to quantify 10 bioactive compounds in Rhus verniciflua extracts using high-performance liquid chromatography-tandem mass spectrometry. The chromatographic separation was performed using a C18 column under gradient elution with 0.1% formic acid and acetonitrile as the mobile phase solvents. The analytes were detected in the negative-ion mode using multiple-reaction monitoring detection with an electrospray ionization interface. The calibration curves for all the analytes showed good linearity (r(2) >0.997) over the concentration range of 1-1,000 ng/mL. The recovery values were within 89.53-110.14%, and the intra- and interday coefficients of variation were <4.86% for all the tested compounds. The developed method was successfully applied to a quality assessment of the R. verniciflua extract samples.
本研究的目的是开发一种同时定量漆树提取物中10种生物活性化合物的方法，采用高效液相色谱-串联质谱法。色谱分离使用C18柱，以0.1%甲酸和乙腈作为流动相溶剂进行梯度洗脱。使用电喷雾电离接口，在负离子模式下通过多反应监测检测分析物。所有分析物的校准曲线在1-1000 ng/mL的浓度范围内显示出良好的线性（r(2)>0.997）。回收率在89.53-110.14%之间，所有测试化合物的日内和日间变异系数均<4.86%。所开发的方法成功应用于漆树提取物样品的质量评估。
