Postic D, Edlinger C, Richaud C, Grimont F, Dufresne Y, Perolat P, Baranton G, Grimont P A
Unité des Leptospires, INSERM U199, Institut Pasteur, Paris.
Res Microbiol. 1990 May;141(4):465-75. doi: 10.1016/0923-2508(90)90072-x.
A total of 13 Borrelia burgdorferi strains (responsible for Lyme borreliosis) and representatives of 3 other Borrelia species (B. hermsii, B. parkeri, B. turicatae) associated with relapsing fever were studied by DNA/DNA hybridization and rRNA gene-restriction patterns. Two genomic DNA hybridization groups were observed which could be differentiated by rRNA gene-restriction patterns. Moreover, the number and size of restriction fragments suggest the existence of a single set of 16 and 23 S rRNA genes in Borrelia.
通过DNA/DNA杂交和rRNA基因限制性图谱，对总共13株伯氏疏螺旋体菌株（引发莱姆病）以及与回归热相关的其他3种疏螺旋体物种（赫氏疏螺旋体、帕克疏螺旋体、杜氏疏螺旋体）的代表菌株进行了研究。观察到两个基因组DNA杂交组，它们可通过rRNA基因限制性图谱加以区分。此外，限制性片段的数量和大小表明疏螺旋体中存在一组单一的16S和23S rRNA基因。
