Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657 8501, Japan.
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657 8501, Japan.
Bioresour Technol. 2013 Nov;147:84-88. doi: 10.1016/j.biortech.2013.07.091. Epub 2013 Jul 25.
Concentrating sugars using membrane separation, followed by ethanol fermentation by recombinant xylose-assimilating Saccharomyces cerevisiae, is an attractive technology. Three nanofiltration membranes (NTR-729HF, NTR-7250, and ESNA3) were effective in concentrating glucose, fructose, and sucrose from dilute molasses solution and no permeation of sucrose. The separation factors of acetate, formate, furfural, and 5-hydroxymethyl furfural, which were produced by dilute acid pretreatment of rice straw, over glucose after passage through these three membranes were 3.37-11.22, 4.71-20.27, 4.32-16.45, and 4.05-16.84, respectively, at pH 5.0, an applied pressure of 1.5 or 2.0 MPa, and 25 °C. The separation factors of these fermentation inhibitors over xylose were infinite, as there was no permeation of xylose. Ethanol production from approximately two-times concentrated liquid hydrolysate using recombinant S. cerevisiae was double (5.34-6.44 g L(-1)) that compared with fermentation of liquid hydrolysate before membrane separation (2.75 g L(-1)).
采用膜分离浓缩糖，然后利用重组木糖同化酿酒酵母进行乙醇发酵，是一种很有吸引力的技术。三种纳滤膜（NTR-729HF、NTR-7250 和 ESNA3）可有效地浓缩稀糖蜜溶液中的葡萄糖、果糖和蔗糖，且蔗糖不会渗透。通过三种膜过滤后，乙酸盐、甲酸盐、糠醛和 5-羟甲基糠醛相对于葡萄糖的分离因子分别为 3.37-11.22、4.71-20.27、4.32-16.45 和 4.05-16.84，在 pH5.0、应用压力为 1.5 或 2.0 MPa 和 25°C 时。这些发酵抑制剂相对于木糖的分离因子是无穷大的，因为木糖不会渗透。与膜分离前的液体水解液发酵（2.75 g L(-1))相比，使用重组酿酒酵母从大约两倍浓缩的液体水解液中生产乙醇的产量增加了一倍（5.34-6.44 g L(-1))。
