Abbi M, Kuhad R C, Singh A
Department of Microbiology, University of Delhi, India.
J Ind Microbiol. 1996 Jul;17(1):20-3. doi: 10.1007/BF01570143.
Candida shehatae NCL-3501 utilized glucose and xylose efficiently in batch cultures. The specific rate of ethanol production was higher with mixtures of glucose and xylose (0.64-0.83 g g-1 cells d-1) compared to that with individual sugars (0.38-0.58 g g-1 cells d-1). Although the optimum temperature for growth was 30 degrees C, this strain grew and produced appreciable levels of ethanol at 45 degrees C. A stable ethanol yield (0.40-0.43 g g-1 substrate utilized) was obtained between 10 g L-1 and 80 g L-1 of initial xylose concentration. Conversion efficiency was further improved by immobilization of the cells in calcium alginate beads. Free or immobilized cells of C. shehatae NCL-3501 efficiently utilized sugars present in rice straw hemicellulose hydrolysate, prepared by two different methods, with 48 h. Ethanol yields of 0.45 g g-1 and 0.5 g g-1 from autohydrolysate, and 0.37 g g-1 from acid hydrolysate were produced by free and immobilized cells, respectively.
嗜杀假丝酵母NCL - 3501在分批培养中能高效利用葡萄糖和木糖。与单独使用糖类（0.38 - 0.58 g g⁻¹细胞 d⁻¹）相比，葡萄糖和木糖混合物的乙醇比生产速率更高（0.64 - 0.83 g g⁻¹细胞 d⁻¹）。尽管该菌株生长的最适温度为30℃，但在45℃时仍能生长并产生可观水平的乙醇。当初始木糖浓度在10 g L⁻¹至80 g L⁻¹之间时，可获得稳定的乙醇产率（0.40 - 0.43 g g⁻¹利用的底物）。通过将细胞固定在海藻酸钙珠中，转化效率进一步提高。嗜杀假丝酵母NCL - 3501的游离或固定化细胞能在48小时内有效利用通过两种不同方法制备的稻草半纤维素水解物中存在的糖类。游离和固定化细胞分别从自水解物中产生了0.45 g g⁻¹和0.5 g g⁻¹的乙醇产率，从酸水解物中产生了0.37 g g⁻¹的乙醇产率。
