Guhsl E E, Hofstetter G, Lengger N, Hemmer W, Ebner C, Fröschl R, Bublin M, Lupinek C, Breiteneder H, Radauer C
Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.
Allergy. 2015 Jan;70(1):59-66. doi: 10.1111/all.12534. Epub 2014 Nov 30.
Birch pollen-associated plant food allergy is caused by Bet v 1-specific IgE, but presence of cross-reactive IgE to related allergens does not predict food allergy. The role of other immunoglobulin isotypes in the birch pollen-plant food syndrome has not been investigated in detail.
Bet v 1-sensitized birch pollen-allergic patients (n = 35) were diagnosed for food allergy by standardized interviews, skin prick tests, prick-to-prick tests and ImmunoCAP. Concentrations of allergen-specific IgE, IgG1, IgG4 and IgA to seven Bet v 1-related food allergens were determined by ELISA.
Bet v 1, Cor a 1, Mal d 1 and Pru p 1 bound IgE from all and IgG4 and IgA from the majority of sera. Immunoglobulins to Gly m 4, Vig r 1 and Api g 1.01 were detected in <65% of the sera. No significant correlation was observed between plant food allergy and increased or reduced levels of IgE, IgG1, IgG4 or IgA specific to most Bet v 1-related allergens. Api g 1-specific IgE was significantly (P = 0.01) elevated in celeriac-allergic compared with celeriac-tolerant patients. Likewise, frequencies of IgE (71% vs 15%; P = 0.01) and IgA (86% vs 38%; P = 0.04) binding to Api g 1.01 were increased.
Measurements of allergen-specific immunoglobulins are not suitable for diagnosing Bet v 1-mediated plant food allergy to hazelnut and Rosaceae fruits. In contrast, IgE and IgA to the distantly related allergen Api g 1 correlate with allergy to celeriac.
桦树花粉相关的植物性食物过敏由Bet v 1特异性IgE引起，但对相关过敏原存在交叉反应性IgE并不能预测食物过敏。其他免疫球蛋白同种型在桦树花粉 - 植物性食物综合征中的作用尚未得到详细研究。
通过标准化访谈、皮肤点刺试验、点刺 - 点刺试验和免疫化学发光法（ImmunoCAP）对35例对Bet v 1致敏的桦树花粉过敏患者进行食物过敏诊断。采用酶联免疫吸附测定法（ELISA）测定七种与Bet v 1相关的食物过敏原的特异性IgE、IgG1、IgG4和IgA浓度。
Bet v 1、Cor a 1、Mal d 1和Pru p 1与所有血清中的IgE以及大多数血清中的IgG4和IgA结合。<65%的血清中检测到针对Gly m 4、Vig r 1和Api g 1.01的免疫球蛋白。大多数与Bet v 1相关的过敏原特异性IgE、IgG1、IgG4或IgA水平的升高或降低与植物性食物过敏之间未观察到显著相关性。与芹菜耐受患者相比，芹菜过敏患者中Api g 1特异性IgE显著升高（P = 0.01）。同样，与Api g 1.01结合的IgE（71%对15%；P = 0.01）和IgA（86%对38%；P = 0.04）的频率也增加。
过敏原特异性免疫球蛋白的检测不适用于诊断Bet v 1介导的对榛子和蔷薇科水果的植物性食物过敏。相比之下，与远缘过敏原Api g 1的IgE和IgA与芹菜过敏相关。
