Hyon Suong-Hyu, Nakajima Naoki, Sugai Hajime, Matsumura Kazuaki
Center for Fiber and Textile Science, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan.
J Biomed Mater Res A. 2014 Aug;102(8):2511-20. doi: 10.1002/jbm.a.34923. Epub 2013 Aug 30.
A novel adhesive hydrogel consisting of dextran and epsilon-poly(L-lysine) (dextran-PL) with multiple biomedical applications was developed. Periodate oxidation in aqueous media almost stoichiometrically introduces aldehyde groups in dextran molecules, and aldehyde dextran can react with the primary amino groups in epsilon-PL (ɛ-PL) at neutral pH to form a hydrogel. The gelation time of the hydrogel can be easily controlled by the extent of oxidation in dextran and of the acylation in ɛ-PL by anhydrides. The shear adhesion strength of dextran-PL was 10 times higher than that of fibrin glue, when wet collagen sheets were selected as test specimens. The cytotoxicity of aldehyde dextran and ɛ-PL were 1000 times lower than that of glutaraldehyde and poly(allylamine). The considerably low cytotoxicity of aldehyde dextran could be ascribed to its low reactivity with amine species when compared with glutaraldehyde. In contrast, a high reactivity of amino groups in ɛ-PL was observed when compared with glycine, L-lysine, and gelatin, which could be explained by their poor dissociation at neutral pH, thus leading to low cytotoxicity.
开发了一种由葡聚糖和ε-聚（L-赖氨酸）（葡聚糖-PL）组成的具有多种生物医学应用的新型粘性水凝胶。在水性介质中进行高碘酸盐氧化几乎以化学计量的方式在葡聚糖分子中引入醛基，并且醛基葡聚糖可以在中性pH下与ε-PL（ɛ-PL）中的伯氨基反应形成水凝胶。水凝胶的凝胶化时间可以通过葡聚糖中的氧化程度和酸酐对ɛ-PL的酰化程度轻松控制。当选择湿胶原片作为测试样品时，葡聚糖-PL的剪切粘附强度比纤维蛋白胶高10倍。醛基葡聚糖和ɛ-PL的细胞毒性比戊二醛和聚（烯丙胺）低1000倍。与戊二醛相比，醛基葡聚糖的细胞毒性相当低可能归因于其与胺类物质的低反应性。相反，与甘氨酸、L-赖氨酸和明胶相比，观察到ɛ-PL中的氨基具有高反应性，这可以通过它们在中性pH下的低解离来解释，从而导致低细胞毒性。
