Pandey Nikhil, Hakamivala Amirhossein, Xu Cancan, Hariharan Prashant, Radionov Boris, Huang Zhong, Liao Jun, Tang Liping, Zimmern Philippe, Nguyen Kytai T, Hong Yi
Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76010, USA.
Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Adv Healthc Mater. 2018 Apr;7(7):e1701069. doi: 10.1002/adhm.201701069. Epub 2017 Dec 4.
Popular bioadhesives, such as fibrin, cyanoacrylate, and albumin-glutaraldehyde based materials, have been applied for clinical applications in wound healing, drug delivery, and bone and soft tissue engineering; however, their performances are limited by weak adhesion strength and rapid degradation. In this study a mussel-inspired, nanocomposite-based, biodegradable tissue adhesive is developed by blending poly(lactic-co-glycolic acid) (PLGA) or N-hydroxysuccinimide modified PLGA nanoparticles (PLGA-NHS) with mussel-inspired alginate-dopamine polymer (Alg-Dopa). Adhesive strength measurement of the nanocomposites on porcine skin-muscle constructs reveals that the incorporation of nanoparticles in Alg-Dopa significantly enhances the tissue adhesive strength compared to the mussel-inspired adhesive alone. The nanocomposite formed by PLGA-NHS nanoparticles shows higher lap shear strength of 33 ± 3 kPa, compared to that of Alg-Dopa hydrogel alone (14 ± 2 kPa). In addition, these nanocomposites are degradable and cytocompatible in vitro, and elicit in vivo minimal inflammatory responses in a rat model, suggesting clinical potential of these nanocomposites as bioadhesives.
常见的生物粘合剂，如纤维蛋白、氰基丙烯酸酯和基于白蛋白-戊二醛的材料，已被应用于伤口愈合、药物递送以及骨和软组织工程的临床应用中；然而，它们的性能受到粘附强度弱和快速降解的限制。在本研究中，通过将聚（乳酸-乙醇酸）（PLGA）或N-羟基琥珀酰亚胺修饰的PLGA纳米颗粒（PLGA-NHS）与受贻贝启发的海藻酸钠-多巴胺聚合物（Alg-Dopa）混合，开发了一种受贻贝启发的、基于纳米复合材料的可生物降解组织粘合剂。对纳米复合材料在猪皮肤-肌肉构建体上的粘附强度测量表明，与单独的受贻贝启发的粘合剂相比，在Alg-Dopa中加入纳米颗粒可显著提高组织粘附强度。与单独的Alg-Dopa水凝胶（14±2 kPa）相比，由PLGA-NHS纳米颗粒形成的纳米复合材料显示出更高的搭接剪切强度，为33±3 kPa。此外，这些纳米复合材料在体外可降解且具有细胞相容性，并且在大鼠模型中引起体内最小的炎症反应，表明这些纳米复合材料作为生物粘合剂具有临床潜力。
