School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan; BMG Incorporated, 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, Japan.
BMG Incorporated, 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, Japan.
Carbohydr Polym. 2022 Feb 15;278:118949. doi: 10.1016/j.carbpol.2021.118949. Epub 2021 Dec 3.
We developed a self-degradable medical adhesive, LYDEX, consisting of periodate-oxidized aldehyde-functionalized dextran (AD) and succinic anhydride-treated ε-poly-l-lysine (SAPL). After gelation and adhesion of LYDEX by Schiff base bond formation between the AD aldehyde groups and SAPL amino groups, molecular degradation associated with the Maillard reaction is initiated, but the detailed degradation mechanism remains unknown. Herein, we elucidated the degradation mechanism of LYDEX by analyzing the main degradation products under typical solution conditions in vitro. The degradation of the LYDEX gel with a sodium periodate/dextran content of 2.5/20 was observed using gel permeation chromatography and infrared and H NMR spectroscopy. The AD ratio in the AD-SAPL mixture increased as the molecular weight decreased with the degradation time. This discovery of LYDEX self-degradability is useful for clarifying other polysaccharide hydrogel degradation mechanisms, and valuable for the use of LYDEX in medical applications, such as hemostatic or sealant materials.
我们开发了一种可自降解的医用胶粘剂 LYDEX，它由高碘酸钠氧化的醛基化葡聚糖（AD）和琥珀酸酐处理的 ε-聚赖氨酸（SAPL）组成。LYDEX 通过 AD 醛基和 SAPL 氨基之间的席夫碱键形成凝胶化和粘附后，与美拉德反应相关的分子降解被启动，但详细的降解机制仍不清楚。在此，我们通过分析体外典型溶液条件下的主要降解产物，阐明了 LYDEX 的降解机制。使用凝胶渗透色谱法、红外和 H NMR 光谱法观察到 2.5/20 钠高碘酸盐/葡聚糖含量的 LYDEX 凝胶的降解。随着降解时间的延长，AD-SAPL 混合物中的 AD 比例随着分子量的降低而增加。这一发现对于阐明其他多糖水凝胶的降解机制非常有用，对于 LYDEX 在止血或密封材料等医学应用中的使用也很有价值。
