Bahadur Amar, Singh U P, Singh D P, Sarma B K, Singh K P, Singh Amitabh, Aust H J
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India.
Mycobiology. 2008 Mar;36(1):60-5. doi: 10.4489/MYCO.2008.36.1.060. Epub 2008 Mar 31.
The effect of methanolic extract of cashewnut (Anacardium occidentale) shell extract was seen on conidial germination of Erysiphe pisi and powdery mildew development in pea (Pisum sativum). Maximum conidial germination inhibition of E. pisi on glass slides was observed at 300 ppm. Similar effect on floated pea leaves was observed after 48 h at the same concentration. Conidial germination on intact untreated pea leaves was also assessed on II and IV nodal leaves while IV and II nodal leaves were treated with the extract and vice versa. There was tremendous reduction in conidial germination on all the nodal leaves. The disease intensity of pea powdery mildew was significantly reduced by methanolic extract of cashewnut shells. Maximum reduction was observed with 200 ppm where 39% disease intensity was recorded in comparison to 96.53% in the control. The phenolic acid content of pea leaves following treatments with this extract varied and no definite pattern was observed. Out of several phenolic compounds, namely, gallic, ferulic, chlorogenic, and cinnamic acids, only gallic acid was found to be present consistently in all the treatments with varied amounts.
研究了腰果(Anacardium occidentale)壳甲醇提取物对豌豆白粉菌(Erysiphe pisi)分生孢子萌发以及豌豆(Pisum sativum)白粉病发展的影响。在玻片上,当浓度为300 ppm时,观察到对豌豆白粉菌分生孢子萌发的抑制作用最大。在相同浓度下处理48小时后,对漂浮的豌豆叶片也观察到类似效果。还在第二和第四节点叶片上评估了完整未处理豌豆叶片上的分生孢子萌发情况,同时用提取物处理第四和第二节点叶片,反之亦然。所有节点叶片上的分生孢子萌发都有大幅减少。腰果壳甲醇提取物显著降低了豌豆白粉病的病害严重程度。在200 ppm时观察到最大降幅,与对照的96.53%相比,病害严重程度记录为39%。用该提取物处理后,豌豆叶片中的酚酸含量有所不同,未观察到明确的模式。在几种酚类化合物中,即没食子酸、阿魏酸、绿原酸和肉桂酸,仅发现没食子酸在所有处理中均持续存在,但其含量各不相同。