Kwon Hyuk Woo, Back In Joung, Ko Han Gyu, You Chang Hyun, Kim Seong Hwan
Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan, Chungnam 330-714, Korea.
Mycobiology. 2008 Mar;36(1):74-6. doi: 10.4489/MYCO.2008.36.1.074. Epub 2008 Mar 31.
To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, β-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.
为了获取香菇（Lentinula edodes）担孢子生化特性的基本信息，采用基于显色平板的测定法评估其产生胞外酶的能力。为此，针对由香菇两个不同亲本双核菌株Sanjo - 101Ho和Sanjo - 108Ho的40个担孢子产生的单核菌株，测试了淀粉酶、纤维二糖酶、β - 葡萄糖苷酶、CM - 纤维素酶、果胶酶、蛋白酶和木聚糖酶。这两个亲本菌株表现出不同程度的胞外酶活性。在两个香菇品种的单核菌株和亲本菌株之间未观察到相同的酶活性程度模式。两个香菇品种的单核菌株之间的胞外酶活性程度也有所不同。我们的结果表明，香菇的双核亲本菌株产生具有非常多样生化特性的单核担孢子。
