Lee Hwa-Yong, Moon Suyun, Shim Donghwan, Hong Chang Pyo, Lee Yi, Koo Chang-Duck, Chung Jong-Wook, Ryu Hojin
Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea.
Department of Biology, Chungbuk National University, Cheongju 28644, Korea.
Genes (Basel). 2017 Mar 24;8(4):109. doi: 10.3390/genes8040109.
The shiitake mushroom () is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly increased, especially in East Asia. Additionally, demand for the development of new cultivars with good agricultural traits has been greatly enhanced, but the development processes are complicated and more challenging than for other edible mushrooms. In this study, we developed 44 novel polymorphic simple sequence repeat (SSR) markers for the determination of shiitake mushroom cultivars based on a whole genome sequencing database of . These markers were found to be polymorphic and reliable when screened in 23 shiitake mushroom cultivars. For the 44 SSR markers developed in this study, the major allele frequency ranged from 0.13 to 0.94; the number of genotypes and number of alleles were each 2-11; the observed and expected heterozygosity were 0.00-1.00 and 0.10-0.90, respectively; and the polymorphic information content value ranged from 0.10 to 0.89. These new markers can be used for molecular breeding, the determination of cultivars, and other applications.
香菇（）是世界上最受欢迎的食用蘑菇之一，因其在医学和药理学方面的价值而备受关注。随着近期先进研究和技术的发展，香菇的农业种植量大幅增加，尤其是在东亚地区。此外，对具有良好农业性状的新品种的开发需求也大大增强，但与其他食用蘑菇相比，其开发过程更为复杂且更具挑战性。在本研究中，我们基于香菇的全基因组测序数据库开发了44个新的多态性简单序列重复（SSR）标记，用于香菇品种的鉴定。当在23个香菇品种中进行筛选时，发现这些标记具有多态性且可靠。对于本研究中开发的44个SSR标记，主要等位基因频率范围为0.13至0.94；基因型数量和等位基因数量均为2至11个；观察到的杂合度和预期杂合度分别为0.00至1.00和0.10至0.90；多态信息含量值范围为0.10至0.89。这些新标记可用于分子育种、品种鉴定及其他应用。
