Nikkon Farjana, Habib M Rowshanul, Karim M Rezaul, Hossain M Shamim, Mosaddik M Ashik, Haque M Ekramul
Department of Biochemistry and Molecular Biology, University of Rajshahi-6205, Bangladesh.
Mycobiology. 2008 Sep;36(3):173-7. doi: 10.4489/MYCO.2008.36.3.173. Epub 2008 Sep 30.
The crude ethanol extracts (stem and fruits), their fractions and two triterpenes, β-Amyrin and 12-Oleanene 3β, 21β-diol, isolated as a mixture from the chloroform soluble fraction of an ethanolic extract of Duranta repens stem, were evaluated for antibacterial, antifungal activities by the disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The structures of the two compounds were confirmed by IR, (1)H-NMR, (13)C-NMR and LC-MS spectral data. The chloroform soluble fraction of stem and ethanol extract of fruits possess potent antishigellosis activity and also exhibited moderate activity against some pathogenic bacteria and fungi but the isolated compound 1 (mixture of β-Amyrin and 12-Oleanene 3β, 21β-diol) showed mild to moderate inhibitory activity to microbial growth. The minimum inhibitory concentrations (MICs) of the extracts (stem and fruits), their fractions and compound 1 were found to be in the range of 32~128 µg/ml. The chloroform soluble fractions of stem and ethanol extract of fruit showed significant cytotoxicity with LC50 value of 0.94 µg/ml and 0.49 µg/ml, respectively against brine shrimp larvae.
对假连翘茎和果实的粗乙醇提取物、其馏分以及从假连翘茎乙醇提取物的氯仿可溶部分中分离得到的两种三萜类化合物β-香树脂醇和12-齐墩果烯-3β,21β-二醇进行了评价，采用纸片扩散法测定其抗菌、抗真菌活性，采用卤虫致死生物测定法测定其细胞毒性。通过红外光谱、（1）H-核磁共振、（13）C-核磁共振和液相色谱-质谱光谱数据确定了这两种化合物的结构。茎的氯仿可溶部分和果实的乙醇提取物具有很强的抗志贺氏菌活性，对一些病原菌和真菌也表现出中等活性，但分离得到的化合物1（β-香树脂醇和12-齐墩果烯-3β,21β-二醇的混合物）对微生物生长表现出轻度至中度的抑制活性。提取物（茎和果实）、其馏分和化合物1的最低抑菌浓度（MIC）在32~128μg/ml范围内。茎的氯仿可溶部分和果实的乙醇提取物对卤虫幼虫具有显著的细胞毒性，LC50值分别为0.94μg/ml和0.49μg/ml。
