Khordad Elnaz, Fazel Alireza, Ebrahimzadeh Bideskan Alireza
Dept. of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran Biomed J. 2013;17(4):206-13. doi: 10.6091/ibj.1229.2013.
Lead toxicity induces retinal cell apoptosis. Vitamin C and garlic may decrease lead-induced apoptosis. This study was undertaken to investigate vitamin C and garlic protective effects on lead-induced apoptosis in eye retina.
Pregnant Wistar rats (n = 72) were divided randomly into 9 groups: (L) treated rats with lead acetate in drinking water and (L+AA) with leaded water and vitamin C intraperitoneally;(L+G), the rats received leaded-water and garlic juice via gavage; (L+AA+G) treated rats with leaded water, ascorbic acid, and garlic juice, (AA) with ascorbic acid, and (G) with garlic juice; (AA+G) treated rats with vitamin C and garlic juice and (Sh) with tap water plus normal hydrogen chloride (HCl) and glucose; normal (N). After 21-day lactation, blood lead level (BLL) in rats was measured, and then their offspring and the rat offspring's eyes were removed and processed for using TUNEL method. TUNEL positive cells in the eye retina were counted and all groups were compared.
BLL increased in L group compared to the control groups and decreased significantly in L + G, L + AA, and L+ AA + G groups compared to L group (P<0.05). TUNELL positive cell number in eye retina significantly increased in L group compared to control groups (P<0.05) and decreased in L+ G, L+ AA, and L+AA + G groups compared to L group (P<0.05).
Garlic juice and ascorbic acid administration during pregnancy and lactation may protect lead-induced apoptosis in rat offspring's eye retina.
铅中毒会诱导视网膜细胞凋亡。维生素C和大蒜可能会减少铅诱导的细胞凋亡。本研究旨在探讨维生素C和大蒜对铅诱导的视网膜细胞凋亡的保护作用。
将72只怀孕的Wistar大鼠随机分为9组：（L）组大鼠饮用含醋酸铅的水，（L+AA）组大鼠饮用含铅水并腹腔注射维生素C；（L+G）组大鼠饮用含铅水并通过灌胃给予大蒜汁；（L+AA+G）组大鼠饮用含铅水、同时给予抗坏血酸和大蒜汁，（AA）组大鼠给予抗坏血酸，（G）组大鼠给予大蒜汁；（AA+G）组大鼠给予维生素C和大蒜汁，（Sh）组大鼠饮用自来水加正常盐酸（HCl）和葡萄糖；正常（N）组。哺乳21天后，测量大鼠的血铅水平（BLL），然后取出其后代及大鼠后代的眼睛，采用TUNEL法进行处理。对视网膜中的TUNEL阳性细胞进行计数，并对所有组进行比较。
与对照组相比，L组的BLL升高，而与L组相比，L+G、L+AA和L+AA+G组的BLL显著降低（P<0.05）。与对照组相比，L组视网膜中的TUNELL阳性细胞数量显著增加（P<0.05），而与L组相比，L+G、L+AA和L+AA+G组的TUNELL阳性细胞数量减少（P<0.05）。
在怀孕和哺乳期间给予大蒜汁和抗坏血酸可能会保护大鼠后代视网膜免受铅诱导的细胞凋亡。
