Li Bailiang, Jin Da, Yu Shangfu, Etareri Evivie Smith, Muhammad Zafarullah, Huo Guicheng, Liu Fei
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
Food Science and Nutrition Unit, Department of Animal Science, Faculty of Agriculture, University of Benin, PMB 1154 Benin City, Nigeria.
Nutrients. 2017 Aug 8;9(8):845. doi: 10.3390/nu9080845.
Lead (Pb) is a toxic contaminating heavy metal that can cause a variety of hazardous effects to both humans and animals. In the present study, subsp. KLDS1.0207 ( KLDS1.0207), which has a remarkable Pb binding capacity and Pb tolerance, was selected for further study. It was observed that the thermodynamic and kinetic model of KLDS1.0207 Pb binding respectively fit with the Langmuir-Freundlich model and the pseudo second-order kinetic model. Scanning electron microscopy and energy dispersive spectroscopy analysis disclosed that the cell surfaces were covered with Pb and that carbon and oxygen elements were chiefly involved in Pb binding. Combined with Fourier transform infrared spectroscopy analysis, it was revealed that the carboxyl, phosphoryl, hydroxyl, amino and amide groups were the main functional groups involved in the Pb adsorption. The protective effects of KLDS1.0207 against acute Pb toxicity in mice was evaluated by prevention and therapy groups, the results in vivo showed that KLDS1.0207 treatment could reduce mortality rates, effectively increase Pb levels in the feces, alleviate tissue Pb enrichment, improve the antioxidant index in the liver and kidney, and relieve renal pathological damage. Our findings show that KLDS1.0207 can be used as a potential probiotic against acute Pb toxicity.
铅（Pb）是一种有毒的污染重金属，会对人类和动物造成多种有害影响。在本研究中，选择了具有显著铅结合能力和铅耐受性的KLDS1.0207亚种（KLDS1.0207）进行进一步研究。观察到KLDS1.0207与铅结合的热力学和动力学模型分别符合Langmuir-Freundlich模型和伪二级动力学模型。扫描电子显微镜和能量色散光谱分析表明，细胞表面覆盖有铅，且碳和氧元素主要参与铅的结合。结合傅里叶变换红外光谱分析，结果显示羧基、磷酰基、羟基、氨基和酰胺基是参与铅吸附的主要官能团。通过预防组和治疗组评估了KLDS1.0207对小鼠急性铅中毒的保护作用，体内实验结果表明，KLDS1.0207处理可降低死亡率，有效提高粪便中的铅含量，减轻组织铅富集，改善肝脏和肾脏中的抗氧化指标，并缓解肾脏病理损伤。我们的研究结果表明，KLDS1.0207可作为一种潜在的益生菌用于对抗急性铅中毒。
