Guinard Jérôme, Prazuck Thierry, Péré Hélène, Poirier Claire, LeGoff Jérôme, Boedec Erwan, Guigon Aurélie, Day Nesrine, Bélec Laurent
Laboratoire de Microbiologie, Centre Hospitalier Régional d'Orléans, Orléans, France.
Int J STD AIDS. 2013 Dec;24(12):944-50. doi: 10.1177/0956462413487328. Epub 2013 Sep 1.
The usefulness of a point-of-care immunochromatographic dual test for the simultaneous detection of both nontreponemal and Treponema pallidum-specific antibodies (Chembio Diagnostics Systems Inc., Medford, NY, USA) was assessed in various situations related to syphilis, by reference to conventional syphilis serology. Thawed sera were obtained from 100 adults including 36 primary syphilis, 6 secondary syphilis, 6 re-infection, 9 recently-treated syphilis, and 43 old syphilis. Doubtful reactivities for the treponemal line were considered positive; doubtful reactivities for the nontreponemal line were considered positive only when the treponemal line was present. The sensitivity, the specificity, and its concordance to gold standard serology of treponemal line were high, around 90%. The sensitivity of nontreponemal line was 96.3%, its specificity 76.7%, and its concordance 83.4%. In conclusion, the dual rapid test from Chembio Diagnostics Systems Inc. is useful for rapid point-of-care diagnosis in the various situations encountered with patients suffering from syphilis.
参照传统梅毒血清学方法，评估了一种即时检测免疫层析双检测法（美国纽约州梅德福市Chembio诊断系统公司）同时检测非梅毒螺旋体和梅毒螺旋体特异性抗体的效用。从100名成年人中获取解冻血清，其中包括36例一期梅毒、6例二期梅毒、6例再感染、9例近期治疗过的梅毒以及43例陈旧性梅毒。梅毒螺旋体检测线的可疑反应性被视为阳性；非梅毒螺旋体检测线的可疑反应性仅在梅毒螺旋体检测线出现时才被视为阳性。梅毒螺旋体检测线的敏感性、特异性及其与金标准血清学的一致性较高，约为90%。非梅毒螺旋体检测线的敏感性为96.3%，特异性为76.7%，一致性为83.4%。总之，Chembio诊断系统公司的双快速检测法对于梅毒患者所遇到的各种情况的即时快速诊断很有用。
