Department of Crop Sciences, University of Illinois, Urbana, IL, USA.
Arch Virol. 2014 Feb;159(2):349-52. doi: 10.1007/s00705-013-1760-1. Epub 2013 Sep 6.
Virulence and double-stranded RNA (dsRNA) profiles of 44 isolates of Fusarium virguliforme were compared. When grouped according to dsRNA profiles, isolates with large dsRNAs were significantly (P≤0.05) less virulent than isolates without dsRNAs. High-throughput sequence analysis of total RNA prepared from cultures with large dsRNAs identified two novel RNA viruses with genome sequences of approximately 9.3 kbp, which were named Fusarium virguliforme dsRNA mycovirus 1 and Fusarium virguliforme dsRNA mycovirus 2. The new viruses were most closely related to a group of unclassified viruses that included viruses of F. graminearum and Phlebiopsis gigantea and are related to members of the family Totiviridae.
比较了 44 株尖孢镰刀菌的毒力和双链 RNA（dsRNA）特征。根据 dsRNA 特征进行分组时，具有大 dsRNA 的分离株的毒力显著（P≤0.05）低于没有 dsRNA 的分离株。从具有大 dsRNA 的培养物中提取的总 RNA 的高通量序列分析鉴定出两种新型 RNA 病毒，其基因组序列约为 9.3 kbp，分别命名为尖孢镰刀菌 dsRNA 真菌病毒 1 和尖孢镰刀菌 dsRNA 真菌病毒 2。新病毒与一组未分类的病毒最为密切相关，其中包括禾谷镰刀菌和 Phlebiopsis gigantea 的病毒，并且与 Totiviridae 家族的成员相关。
