Li Rongpeng, Jiang Yuji, Wang Xinfeng, Yang Jingjing, Gao Yuan, Zi Xiaoli, Zhang Xia, Gao Haofeng, Hu Nan
Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
Springerplus. 2013 Jul 23;2:335. doi: 10.1186/2193-1801-2-335. eCollection 2013.
A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.
从中国长白山采集的土壤样本中分离出一株新型兼性嗜冷菌（CBS-1菌株），该菌株可积累聚-β-羟基丁酸酯（PHB）。基于16S rRNA序列数据的系统发育分析和Biolog分析确定CBS-1菌株为曼氏假单胞菌。透射电子显微镜照片显示细胞内有大量电子透明颗粒。氢核磁共振分析表明这些颗粒由PHB组成。曼氏假单胞菌CBS-1在20°C时生长最佳。以蔗糖为唯一碳源进行好氧培养48小时后，CBS-1菌株的细胞干重最大细胞密度为29.3 g/L，合成了22.3 g/L的PHB。CBS-1菌株在低温下生长并快速合成高水平PHB的能力可能会降低工业生产PHB的成本。
