López Nancy I, Pettinari M Julia, Stackebrandt Erko, Tribelli Paula M, Põtter Markus, Steinbüchel Alexander, Méndez Beatriz S
Dpto. de Qca. Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Curr Microbiol. 2009 Nov;59(5):514-9. doi: 10.1007/s00284-009-9469-9. Epub 2009 Aug 18.
A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3(T) is closely related to Pseudomonas veronii DSM 11331(T), sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, ss-galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37 degrees C but not at 42 degrees C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3(T) therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3(T) has been deposited as DSM 17835(T) and as CIP 109839(T).
从南极洲的一个临时池塘中分离出一株革兰氏阴性、可运动、杆状、不形成芽孢的细菌（菌株14-3(T)）。基于16S rRNA基因序列相似性，菌株14-3(T)被证明属于狭义假单胞菌属。生理生化测试支持了其系统发育归属。菌株14-3(T)与维罗纳假单胞菌DSM 11331(T)密切相关，序列相似性为99.7%。两菌株间的DNA-DNA杂交实验显示只有中等程度的重缔合相似性（35.1%）。精氨酸双水解酶和硝酸盐还原测试呈阳性，而反硝化、吲哚产生、葡萄糖酸化、脲酶、β-半乳糖苷酶、七叶苷、酪蛋白和明胶水解测试呈阴性。该细菌在4至37℃范围内生长，但在42℃时不生长。在辛酸钠培养基上生长时它积累聚（3-羟基丁酸酯）。因此，菌株14-3(T)代表一个新物种的模式菌株，为此提出新名称南极假单胞菌（Pseudomonas extremaustralis sp. nov.）。模式菌株14-3(T)已保藏为DSM 17835(T)和CIP 109839(T)。
