Natarajan Venkatesan, Arul Gnana Dhas Anton Smith
Department of Pharmacy, Annamalai University, Annamalai Nagar, Tamil nadu 608 002 India.
Springerplus. 2013 Aug 21;2:394. doi: 10.1186/2193-1801-2-394. eCollection 2013.
The objective of the present study was to evaluate the effect of active fraction from Dregea volubilis [Linn.] Benth leaves on serum glucose and lipid profile in normal and diabetic rats.
Diabetes was induced by streptozotocin in wistar rats. Petroleum ether, ethyl acetate, and ethanol extracts of Dregea volubilis [Linn.] Benth leaves were administered orally at a dose of 200 mg/kg, p.o. Metformin was used as standard Anti-diabetic drug (50 mg/kg, p.o). The extract showing for higher Anti-diabetic activity was subjected to column chromatography that led to isolation of an active fraction, which was given trivial name Dv-1. Dv-1 (100 mg/kg, p.o.) was studied for its hypoglycemic and hypolipidemic potential.
Ethanol extract was found to lower the Fasting blood glucose (FBG) level significantly (p < 0.05) in diabetic rats. Dv-1 caused a significant (p < 0.05) reduction in FBG level. Additionally it also caused reduction in cholesterol, triglyceride levels and improvement in the HDL level in diabetic rats.
Reduction in the FBG, cholesterol, triglyceride levels and improvement in the HDL by Dv-1 indicates that Dv-1 has Anti-diabetic activity along with anti hyperlipidemic efficacy and provides a scientific rationale for the use as an Anti-diabetic agent.
本研究的目的是评估南山藤叶的活性成分对正常大鼠和糖尿病大鼠血糖及血脂水平的影响。
用链脲佐菌素诱导Wistar大鼠患糖尿病。南山藤叶的石油醚、乙酸乙酯和乙醇提取物按200mg/kg的剂量口服给药。二甲双胍用作标准抗糖尿病药物（50mg/kg，口服）。对显示出较高抗糖尿病活性的提取物进行柱色谱分离，得到一种活性成分，命名为Dv-1。研究了Dv-1（100mg/kg，口服）的降血糖和降血脂潜力。
发现乙醇提取物能显著降低糖尿病大鼠的空腹血糖（FBG）水平（p<0.05）。Dv-1使FBG水平显著降低（p<0.05）。此外，它还能降低糖尿病大鼠的胆固醇、甘油三酯水平，并提高高密度脂蛋白水平。
Dv-1降低FBG、胆固醇、甘油三酯水平并提高高密度脂蛋白水平，表明Dv-1具有抗糖尿病活性和抗高血脂功效，为其作为抗糖尿病药物的应用提供了科学依据。
