Department of Pharmaceutical Sciences, SBS (PG) Institute of Biomedical Sciences, Balawala, Dehradun, India.
Indian J Pharmacol. 2009 Feb;41(1):19-22. doi: 10.4103/0253-7613.48887.
The objective of the present study was to investigate the hypoglycemic and hepatoprotective effect of Cassia glauca leaf extracts on normal and non insulin dependent diabetes mellitus (NIDDM) in rats. The study was further carried out to investigate the effect of different fractions of the active extract of Cassia glauca, on normal and NIDDM rats, and the effect of active fraction on the blood glucose and hepatic enzymes level.
Diabetes was induced by streptozotocin (STZ) at a dose of 90mg/kg, i.p. in neonates. Different extracts of cassia glauca (100mg/kg, p.o.) were administered to the diabetic rats. Acetone extract was found to lower the serum glucose level significantly in diabetic rats. Further, the acetone extract was subjected to column chromatography and four fractions were obtained on the basis of TLC. All the four fractions (100mg/kg, p.o.) were administered to the diabetic rats. Fraction 1 (F1) caused the maximum reduction in the blood glucose level. The results of the test were compared with the standard antidiabetic drug glibenclamide (5mg/kg, p.o.).
Fraction 1 of acetone extract caused a significant reduction in the levels of hepatic enzyme Aspartate transaminase (AST), alanine transaminase (ALT), creatine kinase (CK), and lactate dehydrogenase (LDH) in STZ-induced diabetic rats.
Improvement in the blood sugar level and normalization of liver functions by Cassia glauca indicates that the plant has hepatoprotective potential, along with antidiabetic activity, and it provides a scientific rationale for the use of Cassia glauca as an antidiabetic agent.
本研究旨在探讨番泻叶提取物对正常和非胰岛素依赖型糖尿病（NIDDM）大鼠的降血糖和保肝作用。本研究还进一步研究了番泻叶活性提取物的不同馏分对正常和 NIDDM 大鼠的影响，以及活性馏分对血糖和肝酶水平的影响。
用链脲佐菌素（STZ）（90mg/kg，ip）诱导新生大鼠糖尿病。给糖尿病大鼠给予不同剂量的番泻叶提取物（100mg/kg，po）。结果发现，丙酮提取物可显著降低糖尿病大鼠的血清葡萄糖水平。进一步将丙酮提取物进行柱层析，根据 TLC 得到四个馏分。所有四个馏分（100mg/kg，po）均给予糖尿病大鼠。馏分 1（F1）使血糖水平降低最明显。将测试结果与标准抗糖尿病药物格列本脲（5mg/kg，po）进行比较。
番泻叶丙酮提取物馏分 1 可显著降低 STZ 诱导的糖尿病大鼠肝酶天门冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶（CK）和乳酸脱氢酶（LDH）的水平。
番泻叶能改善血糖水平和肝功能正常化，表明该植物具有保肝潜力，同时具有抗糖尿病活性，为番泻叶作为抗糖尿病药物的应用提供了科学依据。
