Kocisová J, Srám R J
Výzkumný ústav psychiatrický, Praha.
Cesk Farm. 1990 May;39(3):131-3.
A new methodology is reported determining the cytogenetic damage in stimulated human peripheral lymphocytes. The methodology is based on the investigation of the frequency of cells with micronuclei utilizing cytochalasin B. The spontaneous frequency of cells with micronuclei in the group of healthy volunteers (9 females and 3 males) of the mean age of 37.8 years was 18.42 micronuclei/1000 cells. Furthermore, the action of the analgesic agent paracetamol on the frequency of cells with micronuclei was examined; after the dose of 3 X 1000 mg of paracetamol administered within 8 h no increase in the occurrence of micronuclei within 1 week was found. The advantage of the use of the test for the determination of micronuclei with cytochalasin B in the testing of new drugs are discussed.
报告了一种测定受刺激人外周血淋巴细胞细胞遗传学损伤的新方法。该方法基于利用细胞松弛素B对含微核细胞频率的研究。平均年龄为37.8岁的健康志愿者组（9名女性和3名男性）中含微核细胞的自发频率为每1000个细胞18.42个微核。此外，还研究了镇痛药对乙酰氨基酚对含微核细胞频率的影响；在8小时内给予3×1000mg对乙酰氨基酚剂量后，未发现1周内微核发生率增加。讨论了在新药测试中使用细胞松弛素B测定微核试验的优势。
