Kocisová J, Srám R J
Cas Lek Cesk. 1989 Apr 28;128(18):568-70.
The authors introduced a technique for assessment of cells with micronuclei in stimulated human peripheral lymphocytes using cytochalasin B (CB technique). The spontaneous level of cells with micronuclei was assessed in an ageing population group. The group comprised fifteen subjects (12 women and 3 men), mean age 79.7 +/- 6.7 years. The spontaneous level of cells with micronuclei was 33.7 per 1000 evaluated cells, i.e. 3.37%. The authors consider the possibility of introducing the test for assessment of micronuclei in human peripheral lymphocytes using cytochalasin B as an alternative test in the cytogenetic analysis of chromosomal aberrations in human peripheral lymphocytes.
作者介绍了一种使用细胞松弛素B评估人外周血淋巴细胞中微核细胞的技术（CB技术）。在一个老年人群组中评估了微核细胞的自发水平。该组包括15名受试者（12名女性和3名男性），平均年龄79.7±6.7岁。微核细胞的自发水平为每1000个评估细胞中有33.7个，即3.37%。作者认为将使用细胞松弛素B评估人外周血淋巴细胞中微核的试验作为人外周血淋巴细胞染色体畸变细胞遗传学分析中的替代试验是可行的。
