Biophotonics Laboratory, Immunology Frontier Research Center IFReC, Osaka University, Suita, Osaka, Japan.
Biophys J. 2013 Sep 3;105(5):1123-32. doi: 10.1016/j.bpj.2013.07.031.
We show how Raman imaging can be combined with independent but simultaneous phase measurements of unlabeled cells, with the resulting data providing information on how the light is retarded and/or scattered by molecules in the cell. We then show, for the first time to our knowledge, how the chemistry of the cell highlighted in the Raman information is related to the cell quantitative phase information revealed in digital holographic microscopy by quantifying how the two sets of spatial information are correlated. The results show that such a multimodal implementation is highly useful for the convenience of having video rate imaging of the cell during the entire Raman measurement time, allowing us to observe how the cell changes during Raman acquisition. More importantly, it also shows that the two sets of label-free data, which result from different scattering mechanisms, are complementary and can be used to interpret the composition and dynamics of the cell, where each mode supplies label-free information not available from the other mode.
我们展示了如何将拉曼成像与未标记细胞的独立但同时的相位测量相结合，所得数据提供了关于光如何被细胞内分子延迟和/或散射的信息。然后，我们首次展示了细胞内化学物质与数字全息显微镜中定量相位信息之间的关系，通过量化这两组空间信息之间的相关性来揭示这一关系。结果表明，这种多模态实现非常有用，因为它方便了在整个拉曼测量时间内对细胞进行视频速率成像，使我们能够观察到细胞在拉曼采集过程中的变化。更重要的是，它还表明，这两组源自不同散射机制的无标记数据是互补的，可以用于解释细胞的组成和动态，其中每个模式提供了其他模式无法提供的无标记信息。
