Ioannidou Vasiliki, Ioannidis Anastasios, Magiorkinis Emmanouil, Bagos Pantelis, Nicolaou Chryssoula, Legakis Nicolaos, Chatzipanagiotou Stylianos
Department of Biopathology and Clinical Microbiology, Athens Medical School, Aeginition Hospital, Vass, Sophias av, 72-74, Athens 115 28, Greece.
BMC Res Notes. 2013 Sep 8;6:359. doi: 10.1186/1756-0500-6-359.
The molecular epidemiology of C. jejuni and C. coli clinical strains isolated from children with gastroenteritis, was investigated using the multilocus sequence typing method (MLST). This analysis establishes for the first time in Greece and constitutes an important tool for the epidemiological surveillance and control of Campylobacter infection in our country.
The MLST genotypes were compared with those gained by other typing methods (HS-typing, PFGE and FlaA typing) and were also phylogenetically analyzed, in order to uncover genetic relationships.
Among 68 C. jejuni strains, 41 different MLST-Sequence Types (MLST-STs) were found. Fifty six strains or 34 MLST-STs could be sorted into 15 different MLST-Sequence Type Complexes (MLST-STCs), while twelve strains or seven MLST-STs did not match any of the MLST-STCs of the database. Twenty C. coli strains belonged to 14 different MLST-STs. Eleven MLST-STs were classified in the same MLST-STC (828), and three were unclassifiable. There was no significant association between the MLST-STs and the results of the other typing methods.Phylogenetic analysis revealed that some strains, classified to the species of C. jejuni, formed a separate, phylogenetically distinct group. In eight strains some alleles belonging to the taxonomic cluster of C. jejuni, were also detected in C. coli and vice versa, a phenomenon caused by the genetic mosaic encountered inside the genus Campylobacter.
The MLST-ST determination proved to be a very useful tool for the typing as well as the identification of Campylobacter on the species level.
采用多位点序列分型方法（MLST）对从患肠胃炎儿童中分离出的空肠弯曲菌和结肠弯曲菌临床菌株进行分子流行病学研究。该分析在希腊首次开展，是我国弯曲杆菌感染流行病学监测与控制的重要工具。
将MLST基因型与通过其他分型方法（热稳定蛋白分型、脉冲场凝胶电泳和鞭毛蛋白A分型）获得的基因型进行比较，并进行系统发育分析，以揭示遗传关系。
在68株空肠弯曲菌菌株中，发现了41种不同的MLST序列类型（MLST-STs）。56株或34种MLST-STs可分为15种不同的MLST序列类型复合体（MLST-STCs），而12株或7种MLST-STs与数据库中的任何MLST-STCs均不匹配。20株结肠弯曲菌菌株属于14种不同的MLST-STs。11种MLST-STs被归类于同一MLST-STC（828），3种无法分类。MLST-STs与其他分型方法的结果之间无显著关联。系统发育分析表明，一些被归类为空肠弯曲菌的菌株形成了一个单独的、系统发育上不同的组。在8株菌株中，在结肠弯曲菌中也检测到了一些属于空肠弯曲菌分类簇的等位基因，反之亦然，这一现象是由弯曲杆菌属内遇到的遗传镶嵌现象引起的。
MLST-ST测定被证明是弯曲杆菌分型及在种水平上鉴定的非常有用的工具。
