Papadimitriou Konstantinos, Ioannidis Anastasios, Slavko Aleksandra, Chronopoulou Genovefa, Marmaras Nektarios, Pangalis Anastasia, Olntasi Elisavet, Vassilaki Niki, Koufogeorgou Efthymia Ioanna, Kolida Iris, Theodoridis Dimitrios, Chatzipanagiotou Stylianos
Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Laboratory of Clinical Microbiology, School of Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Rimini 1, 12462 Chaidari, Greece.
Microorganisms. 2025 Jun 18;13(6):1420. doi: 10.3390/microorganisms13061420.
and are the two main campylobacter species that cause foodborne campylobacteriosis. Recent studies have reported that spp. are prone to developing resistance to antibiotics commonly used for their treatment, with many strains identified as multidrug-resistant. This study presents the results of the whole-genome sequencing analysis of the multidrug-resistant strain BCT3 isolated in Greece from a stool specimen of a pediatric patient presenting with diarrhea. The strain was isolated using selective culture media and, based on antimicrobial susceptibility tests, was found to be resistant to ciprofloxacin, tetracycline, erythromycin, azithromycin, clarithromycin, and doxycycline. To further characterize it, we performed whole-genome sequencing, which identified strain BCT3 as . Moreover, multilocus sequence typing assigned the BCT3 to the sequence type (ST) 872, belonging to clonal complex ST-828. The presence of multiple virulence genes revealed its pathogenic potential. The detection of antimicrobial resistance genes and mutated alleles was indicative of its resistance to fluoroquinolones, macrolides, and tetracyclines, supporting the observed phenotype. To our knowledge, this is the first reported clinical case of such a multidrug-resistant strain in Greece.
空肠弯曲菌和结肠弯曲菌是导致食源性弯曲菌病的两种主要弯曲菌属菌种。最近的研究报告称，空肠弯曲菌属菌种易于对常用于治疗它们的抗生素产生耐药性，许多空肠弯曲菌菌株被鉴定为多重耐药。本研究展示了从希腊一名腹泻儿科患者粪便标本中分离出的多重耐药空肠弯曲菌菌株BCT3的全基因组测序分析结果。该菌株使用选择性培养基分离得到，基于药敏试验，发现其对环丙沙星、四环素、红霉素、阿奇霉素、克拉霉素和强力霉素耐药。为了进一步对其进行表征，我们进行了全基因组测序，结果确定菌株BCT3为空肠弯曲菌。此外，多位点序列分型将BCT3归为序列型（ST）872，属于克隆复合体ST-828。多个毒力基因的存在揭示了其致病潜力。抗菌耐药基因和突变等位基因的检测表明其对氟喹诺酮类、大环内酯类和四环素类药物耐药，支持了观察到的表型。据我们所知，这是希腊首次报道的此类多重耐药空肠弯曲菌菌株的临床病例。
