Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Russell Research Center, USDA Agricultural Research Service, 950 College Station Rd, Athens, GA 30605, USA.
Microb Ecol. 2013 Jan;65(1):22-7. doi: 10.1007/s00248-012-0117-8. Epub 2012 Sep 4.
Waterways should be considered in the migration routes of Campylobacter, and the genus has been isolated from several water sources. Inferences on migration routes can be made from tracking genetic types in populations found in specific habitats and testing how they are linked to other types. Water samples were taken over a 4-year period from waterways in the Upper Oconee River Watershed, Georgia, to recover isolates of thermophilic Campylobacter. The isolates were typed by multilocus sequence typing (MLST) and analyzed to determine the overall diversity of Campylobacter in that environment. Forty-seven independent isolates were recovered from 560 samples (8.4 %). Two (4 %) isolates were Campylobacter coli, three (6 %) isolates were putatively identified as Campylobacter lari, and the remaining 42 (~90 %) were Campylobacter jejuni. The C. jejuni and C. coli isolates were typed by the Oxford MLST scheme. Thirty sequence types (STs) were identified including 13 STs that were not found before in the MLST database, including 24 novel alleles. Of the 17 previously described STs, 10 have been isolated from humans, 6 from environmental water, and 6 from wild birds (five types from multiple sources). Seven sites had multiple positive samples, and on two occasions, the same ST was isolated at the same site. The most common type was STST61 with four isolates, and the most common clonal complex was CC179 with nine isolates. CC179 has been commonly associated with environmental water. Although some Campylobacter STs that were found in the Oconee River engage in widespread migration, most are tightly associated with or unique to environmental water sources.
水系应被视为弯曲杆菌的迁徙途径，该属已从多个水源中分离出来。通过跟踪特定栖息地中发现的种群的遗传类型，并测试它们与其他类型的联系，可以推断出迁徙途径。在佐治亚州奥克尼河上游流域的 4 年期间，从水道中采集水样，以回收嗜热弯曲杆菌的分离株。通过多位点序列分型 (MLST) 对分离株进行分型，并进行分析以确定该环境中弯曲杆菌的总体多样性。从 560 个样本中回收了 47 个独立的分离株（8.4%）。2 个（4%）分离株为大肠弯曲杆菌，3 个（6%）分离株被推测为拉里弯曲杆菌，其余 42 个（~90%）为空肠弯曲杆菌。空肠弯曲杆菌和大肠弯曲杆菌分离株通过牛津 MLST 方案进行分型。确定了 30 个序列型（ST），包括以前在 MLST 数据库中未发现的 13 个 ST，包括 24 个新等位基因。在以前描述的 17 个 ST 中，有 10 个从人类中分离出来，6 个从环境水中分离出来，6 个从野生鸟类中分离出来（5 个类型来自多个来源）。有 7 个地点有多个阳性样本，有两次在同一地点分离出相同的 ST。最常见的类型是 STST61，有 4 个分离株，最常见的克隆复合体是 CC179，有 9 个分离株。CC179 通常与环境水有关。虽然在奥克尼河中发现的一些弯曲杆菌 ST 具有广泛的迁移能力，但大多数与环境水源密切相关或独特。
