Department of Entomology and Plant Pathology, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes RJ CEP 28013-602, Brazil.
Parasit Vectors. 2013 Sep 8;6(1):256. doi: 10.1186/1756-3305-6-256.
Entomopathogenic fungi could be useful tools for reducing populations of the dengue mosquito Aedes aegypti. Here the efficiency of fungus (Metarhizium anisopliae) impregnated cloths (with and without imidacloprid [IMI]) was evaluated against adult A. aegypti in simulated human dwellings. Behaviour of mosquitoes in the presence of black cloths was also investigated.
When mosquitoes were released into the test rooms, the lowest survival rates (38%) were seen when five black cloths impregnated with conidia of ESALQ 818 + 10 ppm IMI were fixed under tables and chairs. This result was significantly lower than the survival rate recorded when cloths were impregnated with ESALQ 818 alone (44%) or ESALQ 818 + 0.1 ppm IMI (43%). Blood fed A. aegypti had lower landing frequencies on black cloths than sucrose fed insects during the first 24 h following feeding, which may have been due to reduced flight activity. Few mosquitoes (4-5%) were observed to land on the cloths during the hours of darkness. The landing pattern of sucrose-fed mosquitoes on non-treated and fungus-treated cloths was similar.
The synergism between M. anisopliae and IMI significantly reduced Aedes survival in simulated field conditions. The use of fungus impregnated cloths is a promising point source application method for the control of adult A. aegypti.
昆虫病原真菌可以作为减少登革热蚊埃及伊蚊种群的有用工具。本研究评估了浸渍有真菌（金龟子绿僵菌）的布（有和没有吡虫啉[IMI]）对模拟人类居所中成年埃及伊蚊的效果。还研究了蚊子在黑布存在下的行为。
当将蚊子释放到测试室中时，当将五块固定在桌子和椅子下面的浸渍有 ESALQ 818+10 ppm IMI 分生孢子的黑布时，观察到最低的存活率（38%）。这一结果明显低于仅浸渍 ESALQ 818（44%）或 ESALQ 818+0.1 ppm IMI（43%）的布的存活率。与喂食蔗糖的昆虫相比，吸血后的埃及伊蚊在喂食后 24 小时内，在黑布上的降落频率较低，这可能是由于飞行活动减少所致。在黑暗的几个小时里，很少有蚊子（4-5%）观察到降落在布上。喂食蔗糖的蚊子在未处理和真菌处理的布上的降落模式相似。
金龟子绿僵菌和 IMI 的协同作用显著降低了模拟野外条件下埃及伊蚊的存活率。使用浸渍有真菌的布是控制成年埃及伊蚊的一种有前途的点状应用方法。
