Vaccine Institute, 3rd Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, PR China.
The Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, OX1 3SY, UK.
J Gen Virol. 2013 Jan;94(Pt 1):76-80. doi: 10.1099/vir.0.047506-0. Epub 2012 Sep 26.
Analysis of partial hepatitis C virus sequences has revealed many novel genotype 6 variants that cannot be unambiguously classified, which obscure the distinctiveness of pre-existing subtypes. To explore this uncertainty, we obtained genomes of 98.0-98.8% full-length for eight such variants (KM35, QC273, TV257, TV476, TV533, L349, QC271 and DH027) and characterized them using phylogenetic analyses and per cent nucleotide similarities. The former four are closely related phylogenetically to subtype 6k, TV533 and L349 to subtype 6l, QC271 to subtypes 6i and 6j, and DH027 to subtypes 6m and 6n. The former six defined a high-level grouping that comprised subtypes 6k and 6l, plus related strains. The threshold between intra- and inter-subtype diversity in this group was indistinct. We propose that similar results would be seen elsewhere if more intermediate variants like QC271 and DH027 were sampled.
对部分丙型肝炎病毒序列的分析揭示了许多新的基因型 6 变体，这些变体无法明确分类，这使得先前存在的亚型的独特性变得模糊。为了探究这种不确定性，我们获得了 8 种此类变体（KM35、QC273、TV257、TV476、TV533、L349、QC271 和 DH027）的全长 98.0-98.8%的基因组，并通过系统进化分析和核苷酸相似性百分比对其进行了特征描述。前四种变体在系统进化上与 6k 亚型密切相关，TV533 和 L349 与 6l 亚型相关，QC271 与 6i 和 6j 亚型相关，DH027 与 6m 和 6n 亚型相关。前六种变体定义了一个高级分组，包括 6k 和 6l 亚型以及相关株。该组中种内和种间多样性的界限不明显。如果更多的中间变体（如 QC271 和 DH027）被采样，我们预计在其他地方也会出现类似的结果。
