Holck A, Blom H, Granum P E
Norwegian Food Research Institute, As.
Gene. 1990 Jul 2;91(1):107-11. doi: 10.1016/0378-1119(90)90169-r.
In Clostridium perfringens the acid-soluble spore proteins (ASSPs) of the alpha/beta type are encoded by two genes, sspC1 and sspC2. These genes, encoding ASSP C1 and C2 proteins, respectively, were cloned as parts of 3.5-kb genomic fragments. The genes were sequenced and polypeptides of 59 (C1) and 60 (C2) amino acids (aa) were deduced from the nucleotide sequence. The N-terminal Met is removed from the primary translation products in both cases, yielding mature polypeptides with Mrs of 6306 (C1) and 6553 (C2). The aa sequences of the two polypeptides were very similar and showed a closer relationship with C. bifermentans ASSPs than with ASSPs from Bacillus species.
在产气荚膜梭菌中，α/β型的酸溶性芽孢蛋白（ASSPs）由两个基因sspC1和sspC2编码。这两个分别编码ASSP C1和C2蛋白的基因，作为3.5 kb基因组片段的一部分被克隆。对这些基因进行了测序，并从核苷酸序列推导了59个氨基酸（C1）和60个氨基酸（C2）的多肽。在这两种情况下，N端甲硫氨酸都从初级翻译产物中去除，产生了分子量分别为6306（C1）和6553（C2）的成熟多肽。这两种多肽的氨基酸序列非常相似，与双酶梭菌的ASSPs的关系比与芽孢杆菌属的ASSPs的关系更密切。
