Minuk G Y, Gauthier T, Benarroch A
Department of Medicine, University of Manitoba, Winnipeg, Canada.
Hepatology. 1990 Sep;12(3 Pt 1):542-6. doi: 10.1002/hep.1840120315.
Polyamines have been reported to play an important role in stimulating hepatic regeneration after partial hepatectomy. To determine whether changes in systemic or hepatic polyamine concentrations correlate with the extent of the regenerative stimulus, serum and tissue putrescine, spermidine and spermine concentrations were determined in groups of adult male rats (n = 6 to 12/group) 0, 24, 48, and 72 hr after 30%, 70% or 90% partial hepatectomy. Serum putrescine levels were variably increased after partial hepatectomy and did not correlate with hepatic regenerative activity. Serum spermidine levels remained unaltered and spermine levels were undetectable both before and after partial hepatectomy. In hepatic tissue, only putrescine concentrations increased in proportion to the extent of hepatic resection. Moreover, there was a significant correlation between hepatic putrescine concentrations and restitution of liver mass (r = 0.778), DNA synthesis (r = 0.8026) and protein synthesis (r = 0.7034) (p less than 0.05). No significant correlations existed between hepatic spermidine or spermine concentrations and these parameters of hepatic regeneration. The results of this study support the hypothesis that hepatic putrescine plays an important role in stimulating hepatic regeneration after partial hepatectomy.
据报道，多胺在促进部分肝切除术后的肝再生中发挥重要作用。为了确定全身或肝脏多胺浓度的变化是否与再生刺激的程度相关，在成年雄性大鼠组（每组n = 6至12只）中，于30%、70%或90%部分肝切除术后0、24、48和72小时测定血清和组织中的腐胺、亚精胺和精胺浓度。部分肝切除术后血清腐胺水平有不同程度升高，但与肝脏再生活性无关。血清亚精胺水平保持不变，部分肝切除术前和术后均未检测到精胺水平。在肝组织中，只有腐胺浓度与肝切除范围成比例增加。此外，肝脏腐胺浓度与肝脏质量恢复（r = 0.778）、DNA合成（r = 0.8026）和蛋白质合成（r = 0.7034）之间存在显著相关性（p小于0.05）。肝脏亚精胺或精胺浓度与这些肝脏再生参数之间不存在显著相关性。本研究结果支持以下假设：肝脏腐胺在促进部分肝切除术后的肝再生中起重要作用。
