Lee Je-O, Shrestha Bhushan, Kim Tae-Woong, Sung Gi-Ho, Sung Jae-Mo
Department of Applied Biology, Entomopathogenic Fungal Culture Collection, Kangwon National University, Chuncheon 200-701, Korea.
Mycobiology. 2007 Dec;35(4):230-4. doi: 10.4489/MYCO.2007.35.4.230. Epub 2007 Dec 31.
In order to breed a Cordyceps bassiana isolate that stably forms fruiting body in artificial cultivation, isolates derived from subculturing and single spores were tested through mating. From C. bassiana EFCC 783, three subcultured isolates EFCC 2830, EFCC 2831 and EFCC 2832 were obtained and fourteen single conidial isolates were obtained from these three subcultured isolates. Two different morphological types were found in the fourteen single conidial isolates. One type was able to form synnemata and another type was not able to form synnemata. Since switch of morphological type was not observed despite their continuous subculturing, cross was performed between the two types and the formation of fruiting body was examined. Ascospores were obtained from a selected fruiting body formed by hybrid of the cross. Self-cross and combinational cross of the ascospore-derived isolates generated hybrids that stably produce high quality fruiting body in artificial media.
为培育出能在人工培养中稳定形成子实体的球孢白僵菌分离株，对继代培养和单孢子衍生的分离株进行了交配测试。从球孢白僵菌EFCC 783中，获得了三个继代培养分离株EFCC 2830、EFCC 2831和EFCC 2832，并从这三个继代培养分离株中获得了14个单分生孢子分离株。在这14个单分生孢子分离株中发现了两种不同的形态类型。一种类型能够形成分生孢子梗束，另一种类型则不能形成分生孢子梗束。尽管对它们进行了连续继代培养，但未观察到形态类型的转变，于是对这两种类型进行了杂交，并检查了子实体的形成情况。从杂交产生的一个选定子实体中获得了子囊孢子。子囊孢子衍生分离株的自交和组合杂交产生了能在人工培养基中稳定产生高质量子实体的杂种。
