Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, United States of America.
PLoS One. 2013 Aug 28;8(8):e71952. doi: 10.1371/journal.pone.0071952. eCollection 2013.
The current study describes the taxonomic and functional composition of metagenomic sequences obtained from a filamentous microbial mat isolated from the Comau fjord, located in the northernmost part of the Chilean Patagonia. The taxonomic composition of the microbial community showed a high proportion of members of the Gammaproteobacteria, including a high number of sequences that were recruited to the genomes of Moritella marina MP-1 and Colwelliapsycherythraea 34H, suggesting the presence of populations related to these two psychrophilic bacterial species. Functional analysis of the community indicated a high proportion of genes coding for the transport and metabolism of amino acids, as well as in energy production. Among the energy production functions, we found protein-coding genes for sulfate and nitrate reduction, both processes associated with Gammaproteobacteria-related sequences. This report provides the first examination of the taxonomic composition and genetic diversity associated with these conspicuous microbial mat communities and provides a framework for future microbial studies in the Comau fjord.
本研究描述了从智利巴塔哥尼亚最北部的科马乌峡湾中分离出的丝状微生物垫的宏基因组序列的分类和功能组成。微生物群落的分类组成显示出很高比例的γ变形菌门成员，其中包括大量被招募到海洋粘球菌 MP-1 和 Colwelliapsycherythraea 34H 基因组中的序列，表明存在与这两个嗜冷细菌物种相关的种群。群落的功能分析表明，编码氨基酸运输和代谢的基因以及能量产生的比例很高。在能量产生功能中，我们发现了编码硫酸盐和硝酸盐还原的蛋白质编码基因，这两个过程都与γ变形菌门相关序列有关。本报告首次对与这些明显的微生物垫群落相关的分类组成和遗传多样性进行了检查，并为科马乌峡湾未来的微生物研究提供了框架。
