Su Xiaofang, Wei Jianfei, Ren Xiangling, Li Linlin, Meng Xianwei, Ren Jun, Tang Fangqiong
Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
J Biomed Nanotechnol. 2013 Oct;9(10):1776-83. doi: 10.1166/jbn.2013.1671.
In this work, a new glucose amperometric biosensor was developed by directly electrospinning poly(vinyl alcohol)/chitostan nanofibers on the surface of the platinum electrode, in which glucose oxidase (GOD) was effectively immobilized in nanofibers by encapsulation. After been cross-linked in glutaraldehyde vapor and modified with a thin nafion film, the nanofibers (PVA/chitosan/GOD)/nafion electrode was used for glucose amperometric measurements. The electrospun nanofibrous enzyme membrane served as a better sensing element than the casing one due to the unique properties of nanofibers such as the special three-dimensional network structure, large pores, high porosity, and large surface to volume ratios. The as-prepared biosensor showed a wide linear calibration range, low detection limit, and low apparent Michaelis-Menten constant in the glucose determination. The stability, reproducibility and anti-interference capability of biosensor were also presented. Furthermore, the new biosensor was successfully applied to detect glucose in human serum samples.
在本研究中，通过将聚乙烯醇/壳聚糖纳米纤维直接电纺到铂电极表面，制备了一种新型葡萄糖安培生物传感器，其中葡萄糖氧化酶（GOD）通过包封有效地固定在纳米纤维中。在戊二醛蒸气中交联并用薄的Nafion膜修饰后，纳米纤维（PVA/壳聚糖/GOD）/Nafion电极用于葡萄糖安培测量。由于纳米纤维具有特殊的三维网络结构、大孔、高孔隙率和大的表面积与体积比等独特性能，电纺纳米纤维酶膜作为传感元件比包埋型的更好。所制备的生物传感器在葡萄糖测定中显示出宽的线性校准范围、低检测限和低表观米氏常数。还介绍了生物传感器的稳定性、重现性和抗干扰能力。此外，新型生物传感器成功应用于检测人血清样品中的葡萄糖。
